Anti-Interleukin-3 IL3 Antibody
Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB in Human.
Anti-Interleukin-3 IL3 Antibody Info At A Glance
|Product Name||Anti-Interleukin-3 IL3 Antibody
See all IL-3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for IL 3/IL3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. IL 3/IL3 information: Molecular Weight: 17233 MW; Subcellular Localization: Secreted; Tissue Specificity: Activated T-cells, mast cells, natural killer cells.|
|Cite This Product||Anti-Interleukin-3 IL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1423)|
|Similar Products From
|Anti-Interleukin-3 IL3 Antibody may replace the following items: sc 1769|sc 28342|sc 34802|sc 34804|sc 7928.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human IL-3(62-82aa EDQDILMENNLRRPNLEAFNR).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For IL3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3562|
|Species Of This Entry||Human|
|Alternative Names||IL-3|Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Methylation; Dephosphorylation; Acetylation; Oxidation; Reduction; Hydroxylation; Cleavage; Prenylation; Ribosylation|
|Gene Location||5q31.1, on Chromasome 5, gene sequence: NC_000005.10 (132060655..132063204)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL3 (Source: Uniprot.org, NCBI)
|Protein Function||Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.|
|Tissue Specificity||Activated T-cells, mast cells, natural killer cells.|
|Related Diseases||Leukemia; Myeloid Leukemia; Leukemia, Myelocytic, Acute; Neoplasms; Malignant Neoplasms; Malignant Paraganglionic Neoplasm; Inflammation; Immunologic Deficiency Syndromes; Diphtheria; Myeloid Leukemia, Chronic; Tissue Adhesions; Lymphoma; Anemia; Cell Transformation, Neoplastic; Asthma; Autoimmune Diseases; Myeloproliferative Disease; Autoimmune Reaction; Infective Disorder; Dysmyelopoietic Syndromes|
|Related Pathways||Cell Proliferation; Cell Growth; Pathogenesis; Cell Cycle; Cell Death; Secretion; Cell Activation; Immune Response; Cell Development; Cytokine Production; T Cell Activation; Cell Differentiation; Programmed Cell Death; Localization; S Phase; T Cell Proliferation; Angiogenesis; Cell Adhesion; Glycosylation; Hemopoiesis|
|Background||The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.|
|Scientific References||PMID: 3497843 by Dorssers L., et al. Characterization of a human multilineage-colony-stimulating factor cDNA clone identified by a conserved noncoding sequence in mouse interleukin-3.|
PMID: 3127463 by Otsuka T., et al. Isolation and characterization of an expressible cDNA encoding human IL-3. Induction of IL-3 mRNA in human T cell clones.
Our Boster Quality Guarantee for Anti-Interleukin-3 IL3 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human IL-3 Protein 10ng
Lane 2: Recombinant Human IL-3 Protein 5ng
Lane 3: Recombinant Human IL-3 Protein 2.5ng
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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