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Product Info Summary
| SKU: | A00113-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-IL33 Antibody Picoband®
SKU/Catalog Number
A00113-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL33 Antibody Picoband® catalog # A00113-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL33 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00113-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IL33 recombinant protein (Position: A102-M264).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00113-2 is reactive to Il33 in Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
29.5 kDa
Background of Il33
Interleukin 33 (IL-33) is a protein that in humans is encoded by the IL33 gene. The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00113-2 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
ELISA (Cap), 1-5μg/ml, -
Positive Control
WB: rat lung tissue, mouse lung tissue, mouse RAW264.7 whole cell
IHC: mouse spleen tissue, rat lung tissue
Validation Images & Assay Conditions
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Western blot analysis of IL33 using anti-IL33 antibody (A00113-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (A00113-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL33 at approximately 35 kDa. The expected band size for IL33 is at 31 kDa.
Click image to see more details
IHC analysis of IL33 using anti-IL33 antibody (A00113-2).
IL33 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL33 Antibody (A00113-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of IL33 using anti-IL33 antibody (A00113-2).
IL33 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL33 Antibody (A00113-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-IL33 Antibody Picoband® (A00113-2)
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16 Customer Q&As for Anti-IL33 Antibody Picoband®
Question
We are currently using anti-IL33 antibody A00113-2 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-IL33 antibody (A00113-2) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
Question
Do you have a BSA free version of anti-IL33 antibody A00113-2 available?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL33 antibody A00113-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-21
Question
I have attached the WB image, lot number and protocol we used for endothelial cell using anti-IL33 antibody A00113-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-24
Question
Is this A00113-2 anti-IL33 antibody reactive to the isotypes of IL33?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The immunogen of A00113-2 anti-IL33 antibody is E. coli-derived rat IL33 recombinant protein (Position: A102-M264). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-20
Question
We need to test anti-IL33 antibody A00113-2 on rat endothelial cell for research purposes, then I may be interested in using anti-IL33 antibody A00113-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The products we sell, including anti-IL33 antibody A00113-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endothelial cell using anti-IL33 antibody A00113-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-07
Question
My boss were happy with the WB result of your anti-IL33 antibody. However we have observed positive staining in left coronary artery nucleus using this antibody. Is that expected? Could you tell me where is IL33 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
Based on literature, left coronary artery does express IL33. Generally IL33 expresses in nucleus. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012
Boster Scientific Support
Answered: 2018-05-17
Question
I was wanting to use your anti-IL33 antibody for ELISA for rat endothelial cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat endothelial cell identification?
D. Walker
Verified customer
Asked: 2017-10-18
Answer
It shows on the product datasheet, A00113-2 anti-IL33 antibody has been tested for ELISA, IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat endothelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-18
Question
Is a blocking peptide available for product anti-IL33 antibody (A00113-2)?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
We do provide the blocking peptide for product anti-IL33 antibody (A00113-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-06-28
Question
I have a question about product A00113-2, anti-IL33 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Roberts
Verified customer
Asked: 2017-06-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00113-2 anti-IL33 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-07
Question
We ordered your anti-IL33 antibody for WB on brain in a previous project. I am using mouse, and I plan to use the antibody for IHC next. I am interested in examining brain as well as left coronary artery in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
I have checked the website and datasheets of our anti-IL33 antibody and I see that A00113-2 has been tested on mouse in both WB and IHC. Thus A00113-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-05-31
Question
Would anti-IL33 antibody A00113-2 work for ELISA with endothelial cell?
V. Rodriguez
Verified customer
Asked: 2016-05-26
Answer
According to the expression profile of endothelial cell, IL33 is highly expressed in endothelial cell. So, it is likely that anti-IL33 antibody A00113-2 will work for ELISA with endothelial cell.
Boster Scientific Support
Answered: 2016-05-26
Question
We have seen staining in mouse brain. Any tips? Is anti-IL33 antibody supposed to stain brain positively?
S. Edwards
Verified customer
Asked: 2015-07-28
Answer
From what I have seen in literature brain does express IL33. From what I have seen in Uniprot.org, IL33 is expressed in left coronary artery, endothelial cell, adrenal gland trachea, brain, among other tissues. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012
Boster Scientific Support
Answered: 2015-07-28
Question
Does A00113-2 anti-IL33 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Krishna
Verified customer
Asked: 2015-03-04
Answer
As indicated on the product datasheet, A00113-2 anti-IL33 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-04
Question
I see that the anti-IL33 antibody A00113-2 works with ELISA, what is the protocol used to produce the result images on the product page?
J. Patel
Verified customer
Asked: 2014-12-19
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-12-19
Question
We want using your anti-IL33 antibody for r-rno-6811558; pi5p studies. Has this antibody been tested with western blotting on rat lymphaden tissue? We would like to see some validation images before ordering.
V. Moore
Verified customer
Asked: 2014-05-12
Answer
We appreciate your inquiry. This A00113-2 anti-IL33 antibody is validated on rat lymphaden tissue, spleen tissue, lung tissue, mouse spleen tissue. It is guaranteed to work for ELISA, IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-05-12
