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Product Info Summary
| SKU: | M01080-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD63 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01080-1
BM4133 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD63 Rabbit Monoclonal Antibody catalog # M01080-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD63 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01080-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BGG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD63
Reactive Species
M01080-1 is reactive to CD63 in Human
Observed Molecular Weight
26 kDa
Calculated molecular weight
25.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01080-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
IHC: human spleen tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of CD63 expression in A375 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human spleen, using CD63 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-CD63 Rabbit Monoclonal Antibody (M01080-1)
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Customer Q&As
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4 Customer Q&As for Anti-CD63 Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-CD63 Rabbit Monoclonal antibody. However we have been able to see positive staining in skeletal muscle cell membrane using this antibody. Is that expected? Could you tell me where is CD63 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Based on literature, skeletal muscle does express CD63. Generally CD63 expresses in cell membrane. Regarding which tissues have CD63 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, and Muscle, Pubmed ID: 15489334
Ovary, Pubmed ID: 2171551
Placenta, Pubmed ID: 17897319
Platelet, Pubmed ID: 7682577
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-12
Question
We have been able to see staining in human skin. Any tips? Is anti-CD63 Rabbit Monoclonal antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
From what I have seen in literature skin does express CD63. From what I have seen in Uniprot.org, CD63 is expressed in upper lobe of lung, ovary, skin, skeletal muscle, lung muscle, platelet, placenta, liver, among other tissues. Regarding which tissues have CD63 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, and Muscle, Pubmed ID: 15489334
Ovary, Pubmed ID: 2171551
Placenta, Pubmed ID: 17897319
Platelet, Pubmed ID: 7682577
Skeletal muscle, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-09
Question
Our lab used your anti-CD63 Rabbit Monoclonal antibody for WB on upper lobe of lung in the past. I am using human, and We want to use the antibody for IHC next. I would like examining upper lobe of lung as well as skeletal muscle in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
I have checked the website and datasheets of our anti-CD63 Rabbit Monoclonal antibody and I see that M01080-1 has been validated on human in both WB and IHC. Thus M01080-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-08
Question
We are currently using anti-CD63 Rabbit Monoclonal antibody M01080-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
The anti-CD63 Rabbit Monoclonal antibody (M01080-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-23
