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Product Info Summary
| SKU: | M02362 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IMP3 (IGF2BP3) Mouse Monoclonal Antibody [Clone ID: OTI4H5]
SKU/Catalog Number
M02362
Size
100 µl
Description
Boster Bio IGF2BP3 mouse monoclonal antibody,clone OTI4H5. Catalog# M02362. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IMP3 (IGF2BP3) Mouse Monoclonal Antibody [Clone ID: OTI4H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02362)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4H5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human IGF2BP3 (NP_006538) produced in E.coli.
Reactive Species
M02362 is reactive to IGF2BP3 in Human, Mouse, Rat
Observed Molecular Weight
69 kDa
Calculated molecular weight
63.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02362 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IHC: 1:250
Validation Images & Assay Conditions
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Western blot analysis of IGF2BP3 using anti-IGF2BP3 antibody (M02362).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IGF2BP3 antigen affinity purified monoclonal antibody (M02362) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGF2BP3 at approximately 69 kDa. The expected band size for IGF2BP3 is at 69 kDa.
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Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-IGF2BP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-IGF2BP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Specific Publications For Anti-IMP3 (IGF2BP3) Mouse Monoclonal Antibody [Clone ID: OTI4H5] (M02362)
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