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Product Info Summary
| SKU: | M02362-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-IMP3 IGF2BP3 Monoclonal Antibody
SKU/Catalog Number
M02362-1
BM5550 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IMP3 IGF2BP3 Monoclonal Antibody catalog # M02362-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IMP3 IGF2BP3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02362-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEDB-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IMP3 RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
Reactive Species
M02362-1 is reactive to IGF2BP3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
63.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02362-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human PC-3 whole cell, human HepG2 whole cell, human RT4 whole cell
Validation Images & Assay Conditions
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Western blot analysis of IGF2BP3 using anti-IGF2BP3 antibody (M02362-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2BP3 antigen affinity purified monoclonal antibody (Catalog # M02362-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2BP3 at approximately 72 kDa. The expected band size for IGF2BP3 is at 64 kDa.
Specific Publications For Anti-IMP3 IGF2BP3 Monoclonal Antibody (M02362-1)
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Customer Q&As
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3 Customer Q&As for Anti-IMP3 IGF2BP3 Monoclonal Antibody
Question
Does anti-IMP3 Monoclonal antibody M02362-1 work for IP with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
According to the expression profile of leukemic t-cell, IGF2BP3 is highly expressed in leukemic t-cell. So, it is likely that anti-IMP3 Monoclonal antibody M02362-1 will work for IP with leukemic t-cell.
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-IMP3 Monoclonal antibody M02362-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
F. Baker
Verified customer
Asked: 2018-05-24
Answer
The anti-IMP3 Monoclonal antibody (M02362-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-24
Question
I have a question about product M02362-1, anti-IMP3 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Roberts
Verified customer
Asked: 2013-11-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02362-1 anti-IMP3 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-06
