Anti-INDOL1/IDO2 Antibody

IDO2 antibody

Boster Bio Anti-INDOL1/IDO2 Antibody catalog # PA1612. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1612
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, WB

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Product Name

Anti-INDOL1/IDO2 Antibody

View all IDO2 Antibodies

SKU/Catalog Number

PA1612

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-INDOL1/IDO2 Antibody catalog # PA1612. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-INDOL1/IDO2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1612)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human INDOL1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1612 is reactive to IDO2 in Human

Applications

PA1612 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

47.075kDa

Background of IDO2

IDO2 (Indoleamine 2,3-dioxygenase 2), also called INDOLEAMINE 2,3-DIOXYGENASE-LIKE 1 or INDOL1, is an enzyme encoded by the INDOL1 gene which metabolizes tryptophan in the kynurenine pathway. By genomic sequence analysis, the INDOL1 gene is mapped on chromosome 8p12 just downstream of the INDO gene. And its exact cytogenetic location is 8p11.21. By database analysis using INDO as probe, followed by RT-PCR of total RNA from various tissues, IDO2 is cloned by human and mouse INDOL1. INDOL1 catabolizes tryptophan as determined by Kyn production, but unlike INDO, is inhibited by D-1-methyl-tryptophan (D-1MT) but not the L-1MT stereoisomer. The Gene Structure of the INDOL1 has 11 exons and spans 74 kb.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For IDO2 (Source: Uniprot.org, NCBI)

Gene Name

IDO2

Full Name

Indoleamine 2,3-dioxygenase 2

Weight

47.075kDa

Superfamily

indoleamine 2,3-dioxygenase family

Alternative Names

EC 1.13.11.-; IDO2; Ido-2; INDOL1; INDOL1indoleamine-pyrrole 2,3 dioxygenase-like 1; indoleamine 2,3-dioxygenase 2; Indoleamine 2,3-dioxygenase-2; indoleamine 2,3-dioxygenase-like 1 protein; Indoleamine 2,3-dioxygenase-like protein 1; Indoleamine-pyrrole 2,3-dioxygenase-like protein 1 IDO2 INDOL1 indoleamine 2,3-dioxygenase 2 indoleamine 2,3-dioxygenase 2|indoleamine 2,3-dioxygenase-like 1 protein|indoleamine 2,3-dioxygenase-like protein 1|indoleamine-pyrrole 2,3 dioxygenase-like 1|indoleamine-pyrrole 2,3-dioxygenase-like protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IDO2, check out the IDO2 Infographic

IDO2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IDO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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2 Customer Q&As for Anti-INDOL1/IDO2 Antibody

Question

We are currently using anti-INDOL1/IDO2 antibody PA1612 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

The anti-INDOL1/IDO2 antibody (PA1612) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-13

Question

Does this antibody only recognize IDO2? Does this antibody have cross-reactivity with IDO1?

Verified Customer

Verified customer

Asked: 2018-03-13

Answer

This antibody has no cross reactivity with IDO1 (We got negative result in WB).

Boster Scientific Support

Answered: 2018-03-13

Order DetailsPrice
PA1612

100μg

$370
PA1612-10ug

10μg sample (liquid)

$99
PA1612-Biotin

100 μg Biotin conjugated

$570
PA1612-Cy3

100 μg Cy3 conjugated

$570
PA1612-Dylight488

100 μg Dylight488 conjugated

$570
PA1612-Dylight550

100 μg Dylight550 conjugated

$570
PA1612-Dylight594

100 μg Dylight594 conjugated

$570
PA1612-FITC

100 μg FITC conjugated

$570
PA1612-HRP

100 μg HRP conjugated

$570
PA1612-APC

100 μg APC conjugated

$670
PA1612-PE

100 μg PE conjugated

$670
PA1612-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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