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Breast Cancer Regulation
Rabbit Polyclonal INDOL1/IDO2 Antibody. Validated in Flow Cytometry, WB and tested in Human.
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Our Boster Quality Guarantee for Anti-INDOL1/IDO2 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
The following reagents are used to generate the images below for Anti-INDOL1/IDO2 Antibody (PA1612).
Figure 1. Western blot analysis of IDO2 using anti- IDO2 antibody (PA1612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates (positive control), Lane 2: human placenta tissue lysates (positive control), Lane 3: human Caco-2 whole cell lysates (positive control), Lane 4: human PC-3 whole cell lysates (negative control). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IDO2 antigen affinity purified polyclonal antibody (Catalog # PA1612) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47KD. The expected band size for IDO2 is at 47KD.
Figure 2. Flow Cytometry analysis of SiHa cells using anti-IDO2 antibody (PA1612). Overlay histogram showing SiHa cells stained with PA1612 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDO2 Antibody (PA1612, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
*if product is indicated to react with multiple species, protein info is based on the human gene.
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