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Product Info Summary
| SKU: | M06002-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-INDOL1 (IDO2) Mouse Monoclonal Antibody [Clone ID: OTI2B9]
SKU/Catalog Number
M06002-2
Size
100 µl
Description
Boster Bio IDO2 mouse monoclonal antibody,clone OTI2B9. Catalog# M06002-2. Tested in IF, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-INDOL1 (IDO2) Mouse Monoclonal Antibody [Clone ID: OTI2B9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06002-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2B9
Isotype
IgG1
Immunogen
Full length human recombinant protein of human IDO2 (NP_919270) produced in HEK293T cell.
Reactive Species
M06002-2 is reactive to IDO2 in Human
Observed Molecular Weight
47 kDa
Calculated molecular weight
47.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06002-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IF: 1:100
Positive Control
WB:mouse liver tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of IDO2 using anti-IDO2 antibody (M06002-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IDO2 antigen affinity purified monoclonal antibody (M06002-2) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47 kDa. The expected band size for IDO2 is at 45 kDa.
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Immunofluorescent staining of HeLa cells using anti-IDO2 mouse monoclonal antibody (M06002-2).
Specific Publications For Anti-INDOL1 (IDO2) Mouse Monoclonal Antibody [Clone ID: OTI2B9] (M06002-2)
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