Human TNFRSF9/4-1BB ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF9/4-1BB. 96wells/kit, with removable strips.
Human TNFRSF9/4-1BB ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human TNFRSF9/4-1BB ELISA Kit PicoKine™
See all 4-1BB/TNFRSF9/CD137 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Cd137/TNFRSF9 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd137/TNFRSF9 tissue specificity: Expressed on the surface of activated T-cells.|
|Cite This Product||Human TNFRSF9/4-1BB ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0988)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: L24-Q186|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For TNFRSF9 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3604|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor receptor superfamily member 9|
|Alternative Names||4-1BB/TNFRSF9/CD137|4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9|
|Post Tranlational Modifications||Phosphorylation; Demethylation; Biotinylation; Cleavage; Glycosylation; Methylation|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Gene Location||1p36.23, on Chromasome 1, gene sequence: NC_000001.11 (7915871..7941607, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TNFRSF9 (Source: Uniprot.org, NCBI)
|Protein Function||Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.|
|Tissue Specificity||Expressed on the surface of activated T-cells.|
|Related Diseases||Neoplasms; Malignant Neoplasms; Autoimmune Reaction; Autoimmune Diseases; Carcinoma; Inflammation; Neoplasms, Experimental; Melanoma; Lymphoma; Graft-vs-host Disease; Leukemia; Diabetes Mellitus; Virus Diseases; Immunologic Deficiency Syndromes; Neoplasm Metastasis; Diabetes Mellitus, Insulin-dependent; Influenza; Arthritis; Lupus Erythematosus, Systemic|
|Related Pathways||Immune Response; Cell Activation; T Cell Activation; Cell Death; Cell Proliferation; Cytokine Production; Secretion; T Cell Proliferation; Pathogenesis; Cell Division; T Cell Costimulation; Cell Growth; Lymphocyte Proliferation; T Cell Anergy; Inflammatory Response; Localization; Cell Differentiation; Humoral Immune Response; Lymphocyte Activation; Hypersensitivity|
|Background||CD137 is a member of the tumor necrosis factor(TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9(TNFRSF9), 4-1BB and induced by lymphocyte activation(ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.|
|Scientific References||PMID: 8088337 by Alderson M.R., et al. Molecular and biological characterization of human 4-1BB and its ligand.|
PMID: 8262389 by Schwarz H., et al. A receptor induced by lymphocyte activation (ILA): a new member of the human nerve-growth-factor/tumor-necrosis-factor receptor family.
Kit Components And Assay QC Data Details Of Human TNFRSF9/4-1BB ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0988-CAP||96-well plate precoated with anti-Human 4-1BB/TNFRSF9/CD137 antibody||1|
|EK0988-ST||lyophilized recombinant Human 4-1BB/TNFRSF9/CD137 standard||10ng/tube|
|EK0988-DA||biotinylated anti-Human 4-1BB/TNFRSF9/CD137 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0988 Human TNFRSF9/4-1BB ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human TNFRSF9/4-1BB ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human TNFRSF9/4-1BB ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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