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Product Info Summary
| SKU: | A06777 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Inhibin beta-A INHBA Antibody
SKU/Catalog Number
A06777
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Inhibin beta-A INHBA Antibody catalog # A06777. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Inhibin beta-A INHBA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06777)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human INHBA. AA range:377-426
Reactive Species
A06777 is reactive to INHBA in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
47.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06777 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:200 (4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Western Blot analysis of MCF-7, 293 cells using Inhibin β-A Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from 293, MCF-7 cells, using INHBA Antibody.
Specific Publications For Anti-Inhibin beta-A INHBA Antibody (A06777)
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Customer Q&As
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7 Customer Q&As for Anti-Inhibin beta-A INHBA Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Inhibin beta-A antibody A06777. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-04
Question
I see that the anti-Inhibin beta-A antibody A06777 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-14
Question
Is a blocking peptide available for product anti-Inhibin beta-A antibody (A06777)?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We do provide the blocking peptide for product anti-Inhibin beta-A antibody (A06777). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-27
Question
Would anti-Inhibin beta-A antibody A06777 work for IHC with testis?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
According to the expression profile of testis, INHBA is highly expressed in testis. So, it is likely that anti-Inhibin beta-A antibody A06777 will work for IHC with testis.
Boster Scientific Support
Answered: 2019-06-26
Question
My question regarding product A06777, anti-Inhibin beta-A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06777 anti-Inhibin beta-A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-20
Question
We are currently using anti-Inhibin beta-A antibody A06777 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
The anti-Inhibin beta-A antibody (A06777) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-11
Question
I was wanting to use your anti-Inhibin beta-A antibody for IHC for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
P. Johnson
Verified customer
Asked: 2014-08-22
Answer
You can see on the product datasheet, A06777 anti-Inhibin beta-A antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-22
