Mouse Activin A ELISA Kit PicoKine™

Mouse Activin A ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Inhba in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0302
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Product Name

Mouse Activin A ELISA Kit PicoKine™

See all Activin A products

SKU/Catalog Number

EK0302

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Activin A ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Inhba in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Activin A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0302)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: G311-S426

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0302 is reactive to Inhba in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0302 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0302-CAP Anti-Mouse Inhba Pre-coated 96-well strip microplate 1
EK0302-ST Mouse Inhba Standard 2 vials, 10 ng/tube
EK0302-DA Mouse Inhba Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0480.0940.1320.2010.3840.6921.3952.313

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Activin A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4015544038146460
Standard deviation3.049.9224.642.2411.5325.76
CV (%)7.6%6.4%5.6%5.9%7.9%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Activin A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 140343638372.236%
Sample 21551661651711645.83.5%
Sample 344045241947844721.324.7%
*number of samples for each test n=16.

Gene/Protein Information For Inhba (Source: Uniprot.Org, NCBI)

Uniprot ID

Q04998

Gene ID

16323

Gene Name

Inhba

Full Name

Inhibin beta A chain

Weight

47.392kDa

Superfamily

TGF-beta family

Alternative Names

Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1 Inhba|inhibin beta-A|inhibin beta A chain|activin A|activin beta-A chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Inhba, check out the Inhba Infographic

Inhba infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Inhba: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0302 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

He L,Wang X,Cheng D,Xiong Z,Liu X. Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 in the uterus in premature ovarian insufficiency mouse models. Mol Med Rep.2021 Jan;23(1):37.doi:10.3892/mmr.2020.
Species: Mouse
EK0302 usage in article: APP:ELISA, SAMPLE:OVARY AND UTERUS TISSUE, DILUTION:NA

Adenovirus-mediated knockdown of activin A receptor type 2A attenuates immune-induced hepatic fibrosis in mice and inhibits interleukin-17-induced activation of primary hepatic stellate cells

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11 Customer Q&As for Mouse Activin A ELISA Kit PicoKine™

Question

Q: how to thaw whole blood sample for Activin A ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-11-23

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Activin A for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-11-23

Question

What is the concentration of Sulfuric Acid in TMB stop solution for EK0302?

Verified customer

Asked: 2020-07-02

Answer

The concentration of Sulfuric Acid in TMB stop solution for the Mouse Activin A ELISA Kit PicoKine™ (EK0302) is 2%.

Boster Scientific Support

Answered: 2020-07-06

Question

Q: can I use citrate plasma as samples in Mouse Activin A Picokine® ELISA Kit (Catalog # EK0302)?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Activin A causing degradation of its protein structure. Activin A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Activin A ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-12-04

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

K. Lopez

Verified customer

Asked: 2018-12-08

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-12-08

Question

Q: how to proceed with the analysis of ELISA data? I have performed Activin A level in saliva.

Verified Customer

Verified customer

Asked: 2018-08-23

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-23

Question

Which proteins were tested to determine EK0309 has no cross-reactivity with other relevant proteins?

Verified Customer

Verified customer

Asked: 2018-04-05

Answer

We tested the following proteins for EK0309 – ALK-1, Cripto, DAN, and Osteoactivin.

Boster Scientific Support

Answered: 2018-04-05

Question

Q: What is the optimal O.D. value for Activin A ELISA kit? I performed your Activin A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Activin A even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-02-13

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Activin A ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Activin A that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-02-13

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-01-30

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-01-30

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Activin A using the EK0302 Mouse Activin A Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-02-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2017-02-11

Question

Q: can you suggest the dilution ratio of serum samples for detection of Activin A in Mouse saliva? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

M. Adams

Verified customer

Asked: 2016-06-23

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Activin A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2016-06-23

Question

Which protein was the antibody raised against? Keywords: dimer, monomer

Verified Customer

Verified customer

Asked: 2015-02-06

Answer

The antibody was raised against the dimer of Activin A. The dimer has two same epitopes, which enables specific binding of Activin A antibodies and minimal cross-reactivity with its family members and other sources of non-specific binding. The capture antibody and the detection antibody used in EK0302 are the same monoclonal antibodies which combine with the same two epitopes on the dimer.

Boster Scientific Support

Answered: 2015-02-06

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