Rat Activin A ELISA Kit PicoKine™

Rat Activin A ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Inhba in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 12pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0303
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Product Name

Rat Activin A ELISA Kit PicoKine™

See all Activin A products

SKU/Catalog Number

EK0303

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Activin A ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Inhba in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 12pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat Activin A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0303)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: G311-S426

Sensitivity

<12 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0303 is reactive to Inhba in Rat samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0303 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0303-CAP Anti-Rat Inhba Pre-coated 96-well strip microplate 1
EK0303-ST Rat Inhba Standard 2 vials, 10 ng/tube
EK0303-DA Rat Inhba Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0250.0810.1200.2220.4330.7611.4292.365

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Activin A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3115145028138453
Standard deviation1.308.0024.201.568.6925.36
CV (%)4.2%5.3%5.6%5.6%6.3%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Activin A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131312731301.735.7%
Sample 215116514417115710.756.8%
Sample 345041240446243224.535.6%
*number of samples for each test n=16.

Gene/Protein Information For Inhba (Source: Uniprot.Org, NCBI)

Uniprot ID

P18331

Gene ID

29200

Gene Name

Inhba

Full Name

Inhibin beta A chain

Weight

47.406kDa

Superfamily

TGF-beta family

Alternative Names

Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1 Inhba|inhibin subunit beta A|inhibin beta A chain|activin A|activin beta-A chain|inhibin beta A subunit|inhibin beta-A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Inhba, check out the Inhba Infographic

Inhba infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Inhba: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0303 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The Extracts of Pacific Oyster (Crassostrea Gigas) Alleviate Ovarian Functional Disorders of Female Rats with Exposure to Bisphenol a Through Decreasing FSHR Expression in Ovarian Tissues

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7 Customer Q&As for Rat Activin A ELISA Kit PicoKine™

Question

Q: is it okay to use citrate plasma as samples in Rat Activin A Picokine® ELISA Kit (Catalog # EK0303)?

C. Lewis

Verified customer

Asked: 2020-01-30

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Activin A causing disruption of its protein structure. Activin A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Activin A ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-01-30

Question

Do you sell the wells/strips from EK0303 Elisa kit separately without the buffers?

Verified customer

Asked: 2020-01-14

Answer

We sell the complete kit. The only thing that gets sold separately if needed are the standards. We don't really disintegrate our kits and sell as it requires additional time and effort to do that. Also, the components to be saved are of no use to us as they are from particular lot numbers.

Boster Scientific Support

Answered: 2020-01-14

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-06-08

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-06-08

Question

Q: how to analyze ELISA data? I measured Activin A level in saliva.

Verified Customer

Verified customer

Asked: 2018-05-06

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-06

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-09-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-09-24

Question

Q: What is the optimal O.D. value for Activin A ELISA kit? I used your Activin A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Activin A even though the O.D. values are not very high?

R. Wu

Verified customer

Asked: 2016-01-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Activin A ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of Activin A that can be declared/interpreted as positive by the above standard is 12pg/ml.

Boster Scientific Support

Answered: 2016-01-27

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Activin A using the EK0303 Rat Activin A Picokine® ELISA Kit?

L. Anand

Verified customer

Asked: 2015-03-31

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2015-03-31

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