This website uses cookies to ensure you get the best experience on our website.
Rat Activin A ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Activin A. 96wells/kit, with removable strips. Cited in 1 publication(s).
Rat Activin A ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0303 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Rat |
| Sample Type: | cell culture supernates, serum, plasma(heparin, EDTA) and saliva. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <12pg/ml |
| Assay Range: | 15.6pg/ml-1000pg/ml |
Overview
| Product Name | Rat Activin A ELISA Kit PicoKine™
See all Inhba primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0303 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Rat Activin A/INHBA with <12pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and saliva. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Activin A/INHBA tissue specificity: |
| Cite This Product | Rat Activin A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0303) |
Product Specs
| Reactivity/Species | Rat |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum, plasma(heparin, EDTA) and saliva. |
| Sensitivity | <12pg/ml |
| Assay Range | 15.6pg/ml-1000pg/ml |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: G311-S426 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|monoclonal antibody from mouse |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P18331 |
|---|---|
| Gene Name | Inhba |
| Protein Name | Inhibin beta A chain |
| Subcellular Localization | Secreted . |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Rat Activin A ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0303-CAP | 96-well plate precoated with anti-Rat Inhba antibody | 1 |
| EK0303-ST | lyophilized recombinant Rat Inhba standard | 10ng/tube |
| EK0303-DA | biotinylated anti-Rat Inhba antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0303 Rat Activin A ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.081 | 0.120 | 0.222 | 0.433 | 0.761 | 1.429 | 2.365 |
We measured random samples of Rat Activin A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 31 | 151 | 450 | 28 | 138 | 453 |
| Standard deviation | 1.30 | 8.00 | 24.20 | 1.56 | 8.69 | 25.36 |
| CV(%) | 4.2% | 5.3% | 5.6% | 5.6% | 6.3% | 5.6% |
We measured the performance consistency of Rat Activin A ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 31 | 27 | 31 | 30 | 1.73 | 5.7% |
| Sample 2 | 151 | 165 | 144 | 171 | 157 | 10.75 | 6.8% |
| Sample 3 | 450 | 412 | 404 | 462 | 432 | 24.53 | 5.6% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| The Extracts of Pacific Oyster (Crassostrea Gigas) Alleviate Ovarian Functional Disorders of Female Rats with Exposure to Bisphenol a Through Decreasing FSHR Expression in Ovarian Tissues | PubMed ID 25395697 |
Submit your review to [email protected] or to Biocompare.com for an Amazon giftcard.
0 reviews
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0303 Rat Activin A ELISA Kit PicoKine™
1 Related Questions
Order Product (EK0303)
Shipping Information
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
| [email protected] | |
| (888) 466-3604 |
Boster Guarantee
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.
See Details
In stock
Order Product
EK0303
$369.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.