Overview
| Product Name |
Rat Activin A ELISA Kit PicoKine™
See more Activin A products |
| Catalog# |
EK0303 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Rat Activin A ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Inhba in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.. Sensitivity: 12pg/ml. |
| Cite This Product |
Rat Activin A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0303)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
| Sensitivity |
<12 pg/ml |
| Assay Range |
15.6 pg/ml - 1,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: CHO; Immunogen sequence: G311-S426 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|monoclonal antibody from mouse |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Inhba (Source: Uniprot.org, NCBI)
| Uniprot Id | P18331 |
|---|
| NCBI Gene Id | 29200 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Inhba |
|---|
| Protein Name | Inhibin beta A chain |
|---|
| Superfamily | TGF-beta family |
|---|
| Alternative Names | Activin A|Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1 |
|---|
| Molecular Weight | 47406 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Inhba, check out the Inhba Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Inhba: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Inhba infographic
Kit Components And Assay QC Data Details Of Rat Activin A ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0303-CAP |
Anti-Rat Inhba Pre-coated 96-well strip microplate | 1 |
| EK0303-ST | Rat Inhba Standard | 2 vials, 10 ng/tube |
| EK0303-DA | Rat Inhba Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0303 Rat Activin A ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.081 | 0.120 | 0.222 | 0.433 | 0.761 | 1.429 | 2.365 |
We measured random samples of Rat Activin A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 31 | 151 | 450 | 28 | 138 | 453 |
| Standard deviation | 1.30 | 8.00 | 24.20 | 1.56 | 8.69 | 25.36 |
| CV (%) | 4.2% | 5.3% | 5.6% | 5.6% | 6.3% | 5.6% |
We measured the performance consistency of Rat Activin A ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 31 | 31 | 27 | 31 | 30 | 1.73 | 5.7% |
| Sample 2 | 151 | 165 | 144 | 171 | 157 | 10.75 | 6.8% |
| Sample 3 | 450 | 412 | 404 | 462 | 432 | 24.53 | 5.6% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| The Extracts of Pacific Oyster (Crassostrea Gigas) Alleviate Ovarian Functional Disorders of Female Rats with Exposure to Bisphenol a Through Decreasing FSHR Expression in Ovarian Tissues |
PubMed ID 25395697 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0303 Rat Activin A ELISA Kit PicoKine™
7 Related Questions
Question
Q: is it okay to use citrate plasma as samples in Rat Activin A Picokine® ELISA Kit (Catalog # EK0303)?
C. Lewis
Asked: 2020-01-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Activin A causing disruption of its protein structure. Activin A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Activin A ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-01-30
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2018-06-08
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-08
Question
Q: how to analyze ELISA data? I measured Activin A level in saliva.
Verified Customer
Asked: 2018-05-06
Boster Scientific Support
Answered: 2018-05-06
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2016-09-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-09-24
Question
Q: What is the optimal O.D. value for Activin A ELISA kit? I used your Activin A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Activin A even though the O.D. values are not very high?
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Activin A ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of Activin A that can be declared/interpreted as positive by the above standard is 12pg/ml.
Boster Scientific Support
Answered: 2016-01-27
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Activin A using the EK0303 Rat Activin A Picokine® ELISA Kit?
L. Anand
Asked: 2015-03-31
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2015-03-31
Question
Do you sell the wells/strips from EK0303 Elisa kit separately without the buffers?
Answer
We sell the complete kit. The only thing that gets sold separately if needed are the standards. We don't really disintegrate our kits and sell as it requires additional time and effort to do that. Also, the components to be saved are of no use to us as they are from particular lot numbers.
Boster Scientific Support
Answered: 2020-01-14
