This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Nos2 antibody (A00368-4) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
Filter:
Flow Cytometry analysis of HEPA1-6 cells using anti-iNOS/Nos2 antibody (A00368-4).
Overlay histogram showing HEPA1-6 cells stained with A00368-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-iNOS/Nos2 Antibody (A00368-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with A00368-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-iNOS/Nos2 Antibody (A00368-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-iNOS/Nos2 Antibody Picoband®
Nos2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Nos2 antibody was positively detected by WB in:
mouse RAW2647(-LPS) whole cell, mouse RAW2647(+LPS) whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Nos2 antibody was positively detected by FC in:
HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-iNOS/Nos2 Antibody Picoband®
View all NOS2 Antibodies
View all NOS2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse iNOS/Nos2 recombinant protein (Position: I40-L1099).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
130.6 kDa
Background of Nos2
Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.
Cite This Product
Anti-iNOS/Nos2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00368-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
95%
High Homology
Cow
83%
Moderate Homology
Human
82%
Moderate Homology
Sheep
82%
Moderate Homology
Monkey
82%
Moderate Homology
Dog
81%
Moderate Homology
Rabbit
80%
Moderate Homology
Zebrafish
57%
Low homology
Drosophila
47%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Nos2 antibody
Flow Cytometry (FC) protocol for Nos2 antibody
Publications
Application:
Species:
AB4-Loaded Nanomicelle Hydrogel Promotes Targeting of the Dysregulated Diabetic Wound Microenvironment via Coordinated Multistage Repair
Published: 2026-08-14
Journal: Gels
Application: WB
Impact Factor: 6.40
Species: Mouse
PMID: 42644966
4‐(5′‐Dimethylamino)‐Naphthalenesulfonyl‐2(3H)‐Benzoxazolone (W3D) Ameliorated COPD Lung Injury Through Regulating Macrophage Polarization Mediated by Glycolysis
Published: 2026-07-20
Journal: ARCHIV DER PHARMAZIE
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 42474394
Metabolomics reveals glycerophospholipid metabolism as the action mechanism of Jin-Tian-Ge capsules in ameliorating inflammatory pain
Published: 2025-09-08
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 40930294
Photocurrent-Directed Immunoregulation Accelerates Osseointegration through Activating Calcium Influx in Macrophages
Published: 2024-10-04
Journal: ADVANCED FUNCTIONAL MATERIALS
Application: ELISA
Impact Factor: 18.50
Species: Mouse
Tea polyphenol nanoparticles enable targeted siRNA delivery and multi-bioactive therapy for abdominal aortic aneurysms
Published: 2024-08-08
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: IF
Impact Factor: 10.60
Species: Mouse
PMID: 39118143
“Butterfly-effect” nano-platforms for cascade immune amplification and tumor clearance through multi-modal therapy and immune sensitization
Published: 2024-06-13
Journal: CHEMICAL ENGINEERING JOURNAL
Application: WB,IHC
Impact Factor: 13.30
Species: Mouse
Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study
Published: 2024-06-06
Journal: Heliyon
Application: WB
Impact Factor: 3.40
Species: Mouse
PMID: 38952369
SerpinA3N attenuates ischemic stroke injury by reducing apoptosis and neuroinflammation
Published: 2021-12-12
Journal: CNS Neuroscience & Therapeutics
Application: WB
Impact Factor: 5.24
Species: Mouse
PMID: 34897996
2,3,7,8-Tetrachlorodibenzo-p-dioxin-induced aryl hydrocarbon receptor activation enhanced the suppressive function of mesenchymal stem cells against splenocyte proliferation
Published: 2019-08-05
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: WB
Impact Factor: 1.65
Species: Mouse
PMID: 31385165
NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology
Published: 2019-07-18
Journal: Cellular & Molecular Immunology
Application: WB
Impact Factor: 8.21
Species: Mouse
PMID: 31320730
Inhibition of Autophagy in Microglia Alters Depressive-Like Behavior via BDNF Pathway in Postpartum Depression
Published: 2018-10-08
Journal: Frontiers in Psychiatry
Application: WB
Impact Factor: 2.86
Species: Mouse
PMID: 30349488
Review
Have you used Anti-iNOS/Nos2 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-iNOS/Nos2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00368-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026