Tnf antibody (PB9246) performs well in WB and shows reactivity with Mouse.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 11 Publications
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Anti-TNF alpha Antibody Picoband®

Tnf antibody

Reactive SpeciesHostApplication
MouseRabbitWB

SKU: PB9246

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

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Tested Applications

Tnf antibody was positively detected by WB in:

Mouse Kidney Tissue, Mouse Intestine Tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
Mouse
Tested
Rat
Predicted

Product Information

Product Name
Anti-TNF alpha Antibody Picoband®
View all TNF Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TNF alpha recombinant protein (Position: L80-Q227). Mouse TNF alpha shares 79% and 95% amino acid (aa) sequence identity with human and rat TNF alpha, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated Molecular Weight

25.9 kDa

Background of Tnf
TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Cite This Product
Anti-TNF alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9246)

Protocols

Western Blot protocol for Tnf antibody

Publications

Application:
Species:
Thiamet-G ameliorates Parkinson's disease-associated cognitive impairment via increasing O-GlcNAcylation of STING in the microglia
Published: 2025-06-26
Journal: NEUROCHEMISTRY INTERNATIONAL
Application: WB
Impact Factor: 4.00
Species: Mouse
PMID: 40578440
Reduction of TRPV1 expression on neurons due to downregulation of P2X7R in neonatal rat dorsal root ganglion satellite glial cells under co-culture conditions
Published: 2024-08-19
Journal: BIOLOGY OF THE CELL
Application: WB
Impact Factor: 2.40
Species: Rat
PMID: 39159475
Using broadly targeted plant metabolomics technology combined with network pharmacology to explore the mechanism of action of the Yishen Gushu formula in the treatment of postmenopausal osteoporosis in vivo
Published: 2024-06-22
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 4.80
Species: Rat
PMID: 38914151
Anthrahydroquinone‑2,6‑disulfonate attenuates PQ‑induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of the PI3K/AKT/eNOS pathway
Published: 2024-06-10
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 5.70
Species: Rat
PMID: 38874017
Esculin ameliorates obesity-induced insulin resistance by improving adipose tissue remodeling and activating the IRS1/PI3K/AKT/GLUT4 pathway
Published: 2023-09-29
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 37778516
Gallic Acid Alleviates Neuropathic Pain Behaviors in Rats by Inhibiting P2X7 Receptor-Mediated NF-κB/STAT3 Signaling Pathway
Published: 2021-08-25
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.81
Species: Rat
PMID: 34512324
CD38 deficiency up-regulated IL-1β and MCP-1 through TLR4/ERK/NF-κB pathway in sepsis pulmonary injury
Published: 2021-06-11
Journal: MICROBES AND INFECTION
Application: WB
Impact Factor: 2.70
Species: Mouse
PMID: 34098107
Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice
Published: 2019-01-21
Journal: Oxidative Medicine and Cellular Longevity
Application: WB
Impact Factor: 4.87
Species: Mouse
PMID: 30805082
Nuclear factor kappa B regulated monocyte chemoattractant protein-1/chemokine CC motif receptor-2 expressing in spinal cord contributes to the maintenance of cancer-induced bone pain in rats
Published: 2018-07-20
Journal: Molecular Pain
Application: WB
Impact Factor: 3.21
Species: Rat
PMID: 29956585
A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis
Published: 2018-04-06
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 3.83
Species: Rat
PMID: 29681857
Renal ischemia-reperfusion injury attenuated by splenic ischemic preconditioning
Published: 2018-04-01
Journal: European Review for Medical and Pharmacological Sciences
Application: WB
Impact Factor: 2.39
Species: Rat
PMID: 29687873

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Product Promise

We promise Anti-TNF alpha Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
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PB9246
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 11, 2026