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Product Info Summary
| SKU: | PB9246 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TNF alpha Antibody Picoband®
SKU/Catalog Number
PB9246
PB0270 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNF alpha Antibody Picoband® catalog # PB9246. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9246)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TNF alpha recombinant protein (Position: L80-Q227). Mouse TNF alpha shares 79% and 95% amino acid (aa) sequence identity with human and rat TNF alpha, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9246 is reactive to Tnf in Mouse
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.9 kDa
Background of Tnf
TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9246 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Kidney Tissue, Mouse Intestine Tissue
Validation Images & Assay Conditions
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Anti-TNF alpha Picoband antibody, PB9246, Western blotting
All lanes: Anti TNF alpha (PB9246) at 0.5ug/ml
WB: Recombinant Mouse TNF alpha Protein 0.5ng
Predicted bind size: 15KD
Observed bind size: 15KD
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Anti-TNF alpha Picoband antibody, PB9246, Western blotting
All lanes: Anti TNF alpha (PB9246) at 0.5ug/ml
Lane 1: Mouse Kidney Tissue Lysate at 50ug
Lane 2: Mouse Intestine Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 25KD
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Effect of gallic acid on the expression of TACE and TNF-α. (A). The expression of TACE in serum was detected by enzyme-linked immunosorbent assay (F (4, 10) = 89.68, p < 0.001). (B). Detection of the TACE and β -actin protein expression in the DRG by Western blotting. (C). The relative protein expression of TACE (F (4, 10) = 24.12, p < 0.001). (D). The expression of TNF-α mRNA was detected by qRT-PCR using β -actin as the housekeeper gene (F (4, 10) = 174.2, p < 0.001). (E). The expression of TNF-α mRNA was detected by qRT-PCR using GAPDH as the housekeeper gene (F (4, 10) = 22.63, p < 0.001). (F). In the DRG, TNF-α and β -actin protein expression was detected by Western blotting. (G) . The relative protein expression of TNF-α (F (4, 10) = 33.47, p < 0.001). (H). The expression of TNF-α in serum was detected by enzyme-linked immunosorbent assay (F (4, 10) = 212.2, p < 0.001). One-way ANOVA was used to detect the expression of TACE and TNF-α. Each group consisted of eight rats. Data are presented as mean ± SEM. *** p < 0.001 versus the Sham group, ### p < 0.001 versus the CCI group.
Index in PubMed under a CC BY license. PMID: 34512324
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The synthesized GILZ-p inhibited LPS induced inflammatory cytokine expression in Müller cells. Western blot analysis was performed to determine the protein expression levels of pro-IL-1β (A,B) , MCP-1 (C,D) , TNF-α (E,F) , and ICAM-1 (G,H) in Müller cells treated with 1000 ng/ml LPS in combination with different concentrations of GILZ-p (0.01, 0.1, 1, and 10 μM)for 24 h. β-actin was used as the loading control. The results of quantitative analysis, as determined by densitometric analysis, were expressed as relative to β-actin. Data represent the mean ± SE; the Mann–Whitney U -test was used for comparisons between two groups. n = 3 for each group. ∗ P < 0.05, ∗∗ P < 0.01. TNF-α, tumor necrosis factor-alpha; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1.
Index in PubMed under a CC BY license. PMID: 29681857
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The synthesized GILZ-p decreased LPS induced inflammatory cytokine secretion in culture medium of Müller cells. The Enzyme-Linked Immunosorbent Assays (ELISA) were performed to determine the protein expression levels of IL-1β, MCP-1, TNF-α, and ICAM-1 in Müller cells treated with 1000 ng/ml LPS in combination with different concentrations of GILZ-p (0.01, 0.1, 1, and 10 μM) for 24 h. Data represent the mean ± SE; the Mann–Whitney U -test was used for comparisons between two groups. n = 6 for each group. ∗ P < 0.05, ∗∗ P < 0.01.
Index in PubMed under a CC BY license. PMID: 29681857
Specific Publications For Anti-TNF alpha Antibody Picoband® (PB9246)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-TNF alpha Antibody Picoband®
Question
Which anti-TNF-alpha antibody is suitable with mouse colon tissue sample and RAW264.7 cells lysate in WB?
Verified customer
Asked: 2019-11-05
Answer
We recommend the Anti-TNF alpha Antibody Picoband PB9246 for the samples.
Boster Scientific Support
Answered: 2019-11-06
Question
We have been able to see staining in mouse leukocyte. Any tips? Is anti-TNF alpha antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
According to literature leukocyte does express TNF. According to Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2019-06-26
Question
We are currently using anti-TNF alpha antibody PB9246 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on pig tissues as well?
S. Li
Verified customer
Asked: 2019-02-07
Answer
The anti-TNF alpha antibody (PB9246) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-07
Question
We want using your anti-TNF alpha antibody for glucose metabolic process studies. Has this antibody been tested with western blotting on mouse kidney tissue? We would like to see some validation images before ordering.
A. Mitchell
Verified customer
Asked: 2018-05-09
Answer
We appreciate your inquiry. This PB9246 anti-TNF alpha antibody is tested on mouse kidney tissue, tissue lysate, intestine tissue. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-09
Question
Our team were satisfied with the WB result of your anti-TNF alpha antibody. However we have observed positive staining in prostatic carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?
R. Gonzalez
Verified customer
Asked: 2014-11-17
Answer
From what I have seen in literature, prostatic carcinoma does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2014-11-17
