radio
SKU MA1053
Size 100μg/vial
Reactivity Human, Pig
Clonality Monoclonal
Clone Number SY5
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-Involucrin Antibody (Monoclonal, SY5)
SKU/Catalog Number MA1053
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Involucrin, involucrin (IVL) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;pig. No cross reactivity with other proteins.
Cite This Product Anti-Involucrin Antibody (Monoclonal, SY5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1053)
Host Mouse
Immunogen Human involucrin.
Reactivity Human, Pig

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, By Heat
Immunocytochemistry , 1μg/ml, Human, pig, -
Immunohistochemistry(Frozen Section), 0.4-1μg/ml, Human, pig, -
Western blot, 2μg/ml, Human, pig

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Images And Assay Conditions

/antibody/ma1053 1 IHC anti involucrin antibody monoclonal sy5.jpg

Anti- -Involucrin antibody, MA1053, IHC(P)
IHC(P): Human Lung Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P48998
Gene Name Ivl
Protein Name Involucrin
Tissue Specificity Keratinocytes of epidermis and other stratified squamous epithelia.
Alternative Names Involucrin;Ivl;
Subcellular Localization Cytoplasm. Constituent of the scaffolding of the cornified envelope.
Molecular Weight 67021 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Background Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.

Order Product (MA1053)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.