Anti-Involucrin Ivl Antibody (Monoclonal, SY5)

Product Name

Anti-Involucrin Ivl Antibody (Monoclonal, SY5)

View all Involucrin Antibodies

SKU/Catalog Number

MA1053

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Involucrin Ivl Antibody (Monoclonal, SY5) catalog # MA1053. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Pig.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Involucrin Ivl Antibody (Monoclonal, SY5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1053)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

SY5

Isotype

Mouse IgG1

Immunogen

Human involucrin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1053 is reactive to IVL in Human, Pig

Applications

MA1053 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

68.479kDa

Background of Involucrin

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, By Heat
Immunocytochemistry , 1μg/ml, Human, pig, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, pig, -
Western blot, 1μg/ml, Human, pig

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Involucrin using anti-Involucrin antibody (MA1053).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Involucrin antigen affinity purified monoclonal antibody (Catalog # MA1053) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Involucrin at approximately 68 kDa. The expected band size for Involucrin is at 68 kDa.

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Figure 2. IHC analysis of Involucrin using anti-Involucrin antibody (MA1053).
Involucrin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Involucrin Antibody (MA1053) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For IVL (Source: Uniprot.org, NCBI)

Alternative Names

involucrin IVL involucrin involucrin

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