Anti-Involucrin Antibody (Monoclonal, SY5)
|Ig Isotype||Mouse IgG1|
|Applications||IHC, ICC, WB|
|Product Name||Anti-Involucrin Antibody (Monoclonal, SY5)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Involucrin, involucrin (IVL) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;pig. No cross reactivity with other proteins.|
|Cite This Product||Anti-Involucrin Antibody (Monoclonal, SY5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1053)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, By Heat
Immunocytochemistry , 1μg/ml, Human, pig, -
Immunohistochemistry(Frozen Section), 0.4-1μg/ml, Human, pig, -
Western blot, 2μg/ml, Human, pig
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
Images And Assay Conditions
Anti- -Involucrin antibody, MA1053, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||Keratinocytes of epidermis and other stratified squamous epithelia.|
|Subcellular Localization||Cytoplasm. Constituent of the scaffolding of the cornified envelope.|
|Molecular Weight||67021 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.|
|Research Areas||Human, Pig
*You can search these to find other products in these research areas.
|Background||Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact email@example.com
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to firstname.lastname@example.org.