Anti-IP10/CXCL10 Antibody

Boster Bio Anti-IP10/CXCL10 Antibody catalog # PA1396. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: PA1396
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-IP10/CXCL10 Antibody

See all CXCL10/IP-10/CRG-2 products

SKU/Catalog Number

PA1396

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IP10/CXCL10 Antibody catalog # PA1396. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IP10/CXCL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1396)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human IP10 (79-98aa SKAIKNLLKAVSKERSKRSP).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1396 is reactive to CXCL10 in Human

Applications

PA1396 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CXCL10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02778

Gene ID

3627

Gene Name

CXCL10

Full Name

C-X-C motif chemokine 10

Weight

10.881kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL10, check out the CXCL10 Infographic

CXCL10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1396 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Qin XJ,Lin X,Xue G,Fan HL,Wang HY,Wu JF,Pei D.CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer.Biosci Rep.2020 Dec 21:BSR20203459.doi: 10.1042/ BSR20203459.Epub ahead of print.PMID:33345267.
Species: Human
PA1396 usage in article: APP:IHC, SAMPLE:PTC TISSUE, DILUTION:1:100

A correlation study between gene polymorphism of Th cell expressed chemokine receptor CXCR3 and its ligand levels with HCV infection prognosis

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17 Customer Q&As for Anti-IP10/CXCL10 Antibody

Question

Would PA1396 anti-IP10/CXCL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

As indicated on the product datasheet, PA1396 anti-IP10/CXCL10 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-28

Question

We need to test anti-IP10/CXCL10 antibody PA1396 on human foreskin keratinocyte for research purposes, then I may be interested in using anti-IP10/CXCL10 antibody PA1396 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

The products we sell, including anti-IP10/CXCL10 antibody PA1396, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-27

Question

I was wanting to use your anti-IP10/CXCL10 antibody for WB for human foreskin keratinocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human foreskin keratinocyte identification?

C. Miller

Verified customer

Asked: 2020-02-18

Answer

As indicated on the product datasheet, PA1396 anti-IP10/CXCL10 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human foreskin keratinocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-18

Question

Will anti-IP10/CXCL10 antibody PA1396 work for WB with foreskin keratinocyte?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

According to the expression profile of foreskin keratinocyte, CXCL10 is highly expressed in foreskin keratinocyte. So, it is likely that anti-IP10/CXCL10 antibody PA1396 will work for WB with foreskin keratinocyte.

Boster Scientific Support

Answered: 2020-02-05

Question

Is a blocking peptide available for product anti-IP10/CXCL10 antibody (PA1396)?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

We do provide the blocking peptide for product anti-IP10/CXCL10 antibody (PA1396). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-06

Question

Is this PA1396 anti-IP10/CXCL10 antibody reactive to the isotypes of CXCL10?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

The immunogen of PA1396 anti-IP10/CXCL10 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human IP10(79-98aa SKAIKNLLKAVSKERSKRSP). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-11

Question

We have been able to see staining in human foreskin keratinocyte. Any tips? Is anti-IP10/CXCL10 antibody supposed to stain foreskin keratinocyte positively?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

From what I have seen in literature foreskin keratinocyte does express CXCL10. From what I have seen in Uniprot.org, CXCL10 is expressed in ectocervix, prostate, keratinocyte, foreskin keratinocyte, among other tissues. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-11-25

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for foreskin keratinocyte using anti-IP10/CXCL10 antibody PA1396. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-08

Question

Our team were content with the WB result of your anti-IP10/CXCL10 antibody. However we have seen positive staining in prostate secreted. using this antibody. Is that expected? Could you tell me where is CXCL10 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

According to literature, prostate does express CXCL10. Generally CXCL10 expresses in secreted. Regarding which tissues have CXCL10 expression, here are a few articles citing expression in various tissues:
Foreskin keratinocyte, Pubmed ID: 11559369
Keratinocyte, Pubmed ID: 10233762
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-29

Question

We are currently using anti-IP10/CXCL10 antibody PA1396 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

The anti-IP10/CXCL10 antibody (PA1396) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-27

Question

Would anti-IP10/CXCL10 antibody PA1396 work on zebrafish IHC with ectocervix?

Verified Customer

Verified customer

Asked: 2018-05-30

Answer

Our lab technicians have not tested anti-IP10/CXCL10 antibody PA1396 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IP10/CXCL10 antibody PA1396 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish ectocervix in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-30

Question

Do you have a BSA free version of anti-IP10/CXCL10 antibody PA1396 available?

Verified Customer

Verified customer

Asked: 2018-05-25

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IP10/CXCL10 antibody PA1396 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-05-25

Question

We ordered your anti-IP10/CXCL10 antibody for WB on ectocervix in a previous project. I am using human, and I plan to use the antibody for IHC next. you antibody examining ectocervix as well as keratinocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

I have checked the website and datasheets of our anti-IP10/CXCL10 antibody and I see that PA1396 has been validated on human in both WB and IHC. Thus PA1396 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-03-19

Question

I see that the anti-IP10/CXCL10 antibody PA1396 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-08

Question

I have a question about product PA1396, anti-IP10/CXCL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1396 anti-IP10/CXCL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-11

Question

I am looking for using your anti-IP10/CXCL10 antibody for adenylate cyclase-activating g protein-coupled receptor signaling pathway studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.

J. Miller

Verified customer

Asked: 2017-09-01

Answer

We appreciate your inquiry. This PA1396 anti-IP10/CXCL10 antibody is tested on mammary cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-09-01

Question

See attached the WB image, lot number and protocol we used for foreskin keratinocyte using anti-IP10/CXCL10 antibody PA1396. Please let me know if you require anything else.

V. Jha

Verified customer

Asked: 2015-03-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-03-30

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