Human CXCL10/IP-10 ELISA Kit PicoKine™

Human CXCL10/IP-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 1pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: EK0735
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Product Name

Human CXCL10/IP-10 ELISA Kit PicoKine™

See all CXCL10/IP-10/CRG-2 products

SKU/Catalog Number

EK0735

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CXCL10/IP-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CXCL10/IP-10 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0735)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V22-P98

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0735 is reactive to CXCL10 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

EK0735 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0735-CAP Anti-Human CXCL10 Pre-coated 96-well strip microplate 1
EK0735-ST Human CXCL10 Standard 2 vials, 10 ng/tube
EK0735-DA Human CXCL10 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0480.0860.1480.2020.3250.5471.0431.884

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CXCL10/IP-10/CRG-2, Dilution ratio of 1:1, concentration in serum is 80-133pg/ml.

Some articles we found to cite concentrations of CXCL10/IP-10/CRG-2 in samples: 18760981, 24120067 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CXCL10/IP-10 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6024387964223865
Standard deviation3.2419.1945.74.2819.447.57
CV (%)5.4%7.9%5.2%6.7%8.7%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL10/IP-10/CRG-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 160546064593.576%
Sample 22432582582602546.832.6%
Sample 387992384897890748.895.3%
*number of samples for each test n=16.

Gene/Protein Information For CXCL10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02778

Gene ID

3627

Gene Name

CXCL10

Full Name

C-X-C motif chemokine 10

Weight

10.881kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL10, check out the CXCL10 Infographic

CXCL10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0735 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang Z,Ao X,Shen Z,Ao L,Wu X,Pu C,Guo W,Xing W,He M,Yuan H,Yu J,Li L,Xu X.TNF-α augments CXCL10/CXCR3 axis activity to induce Epithelial-Mesenchymal Transition in colon cancer cell.Int J Biol Sci 2021;17(11):2683-2702.doi:10.7150/ijbs.61350.Available from https://www.ijbs.com/v17p2683.htm
Species: Human
EK0735 usage in article: APP:ELISA, SAMPLE:SW480 CELL AND SW620 CELL, DILUTION:NA

Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China

The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts

Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...

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3 Customer Q&As for Human CXCL10/IP-10 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

C. Chakrabarti

Verified customer

Asked: 2020-05-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-05-12

Question

Q: how to analyze ELISA data? I have performed CXCL10 level in serum.

Verified Customer

Verified customer

Asked: 2018-08-22

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-08-22

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-04-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-04-17

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