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Product Info Summary
| SKU: | PB9022 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband®
SKU/Catalog Number
PB9022
PB0024 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Chemokine signaling: CCL3 → CCR1/CCR5; leukocyte recruitment in innate/adaptive responses. Assay context: Picoband® rabbit polyclonal validated for ELISA/WB (rat). Frequently paired with Th17 axis IL-17A for inflammatory profiling and with endothelial dysfunction readout ESM1/Endocan; may be examined against xenobiotic–immune crosstalk via AHR (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9022)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat CCL3 recombinant protein (Position: A24-A92). Rat CCL3 shares 74% and 91% amino acid (aa) sequences identity with human and mouse CCL3, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9022 is reactive to Ccl3 in Rat
Observed Molecular Weight
33 kDa
Calculated molecular weight
10.3 kDa
Background of Ccl3
Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for CCL3 predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. CCL3 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. CCL3 is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9022 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Western blot analysis of CCL3 using anti-CCL3 antibody (PB9022).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Rat CCL3 Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9022) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 33KD. The expected band size for CCL3 is at 33KD.
Specific Publications For Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband® (PB9022)
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