Human CXCL10/IP-10 PicoKine™ Quick ELISA Kit

Human CXCL10/IP-10 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 1pg/ml.

Product Info Summary

SKU: FEK0735
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
Assay Time: 90 Minutes

Product Name

Human CXCL10/IP-10 PicoKine™ Quick ELISA Kit

See all CXCL10/IP-10/CRG-2 products

SKU/Catalog Number

FEK0735

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CXCL10/IP-10 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CXCL10 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CXCL10/IP-10 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0735)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V22-P98

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0735 is reactive to CXCL10 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine

Application Guarantee

FEK0735 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK0735-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0735-STHuman CXCL10/IP-10/CRG-2 Standard 2 10 ng/tube
FEK0735-CHuman CXCL10 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0250.0940.1450.2520.4300.7591.3882.144

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6624589469236874
Standard deviation3.6318.6248.284.5520.7744.57
CV (%)5.5%7.6%5.4%6.6%8.8%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL10/IP-10/CRG-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 166556158603.545.9%
Sample 22452632692542587.222.8%
Sample 389493584297191149.175.4%
*number of samples for each test n=16.

Gene/Protein Information For CXCL10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02778

Gene ID

3627

Gene Name

CXCL10

Full Name

C-X-C motif chemokine 10

Weight

10.881kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 CXCL10 C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1 C-X-C motif chemokine ligand 10 C-X-C motif chemokine 10|10 kDa interferon gamma-induced protein|gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine subfamily B (Cys-X-Cys), member 10|small-inducible cytokine B10

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL10, check out the CXCL10 Infographic

CXCL10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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