Product Info Summary
SKU: | A01247-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IRAK-4 Antibody
SKU/Catalog Number
A01247-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IRAK-4 Antibody (Catalog # A01247-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
IRAK-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IRAK-4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01247-1)
Host
Rabbit
Contents
IRAK-4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IRAK4 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK4. The immunogen is located within the last 50 amino acids of IRAK4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.
Reactive Species
A01247-1 is reactive to IRAK4 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
51530 MW
Background of IRAK4
Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-κB and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-κB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01247-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; ICC: 10 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western Blot Validation in Human Cell Lines
Loading: 15 μg/ of lysates per lane.Antibodies: IRAK4 A01247-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: IRAK4-A01247-1 (1 μg/mL), IRAK4 (1 μg/mL), beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human HeLa Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: IRAK4 A01247-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 μg/mLLane 2: 2 μg/mLLane 3: 4 μg/mL
Click image to see more details
Immunocytochemistry Validation of IRAK4 in K562 Cells
Immunocytochemical analysis of K562 cells using anti-IRAK4 antibody (A01247-1) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Immunofluorescence Validation of IRAK4 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling IRAK4 with A01247-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Click image to see more details
KO and KD Validation of IRAK4 in Mouse Bone Marrow-derived Macrophages (BMDM) (Koziczak-Holbro et al.
Click image to see more details
Regulated Expression Validation of IRAK4 in Mouse RAW 264 Cells (Hatao et al.
Click image to see more details
KD Validation of IRAK4 in HEK293T Cells (Heinz et al.
Protein Target Info & Infographic
Gene/Protein Information For IRAK4 (Source: Uniprot.org, NCBI)
Gene Name
IRAK4
Full Name
Interleukin-1 receptor-associated kinase 4
Weight
51530 MW
Superfamily
protein kinase superfamily
Alternative Names
IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64 IRAK4 IMD67, IPD1, IRAK-4, NY-REN-64, REN64 interleukin 1 receptor associated kinase 4 interleukin-1 receptor-associated kinase 4|renal carcinoma NY-REN-64
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRAK4, check out the IRAK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRAK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRAK-4 Antibody (A01247-1)
Hello CJ!
No publications found for A01247-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IRAK-4 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IRAK-4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question