Product Info Summary
SKU: | PA1942 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-IRAK4 Antibody Picoband®
SKU/Catalog Number
PA1942
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRAK4 Antibody catalog # PA1942. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRAK4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1942)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK4, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1942 is reactive to IRAK4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
89 kDa
Calculated molecular weight
51530 MW
Background of IRAK4
IRAK-4 (interleukin-1 receptor-associated kinase 4), also called REN64, in the IRAK family, is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors. Scott (2002) mapped the REN64/IRAK4 gene to chromosome 12 based on similarity between the REN64 sequence (GenBank AF155118) and a chromosome 12 clone (GenBank AC093012). Functional analysis by Li et al. (2002) determined that IRAK4, like IRAK1 and Pelle, has auto- and cross-phosphorylation kinase activity. Precipitation and binding analyses showed weak interaction between IRAK4 and IRAK1, but IRAK4 did not interact with other IRAK family members. Overexpressed IRAK4 interacted with MYD88 and TRAF6 and activated mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NFKB) pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1942 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA Cell, U87 Cell, MCF-7 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-IRAK4 antibody, PA1942, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Lane 3: MCF-7 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For IRAK4 (Source: Uniprot.org, NCBI)
Gene Name
IRAK4
Full Name
Interleukin-1 receptor-associated kinase 4
Weight
51530 MW
Superfamily
protein kinase superfamily
Alternative Names
Interleukin-1 receptor-associated kinase 4;IRAK-4;2.7.11.1;Renal carcinoma antigen NY-REN-64;IRAK4; IRAK4 IMD67, IPD1, IRAK-4, NY-REN-64, REN64 interleukin 1 receptor associated kinase 4 interleukin-1 receptor-associated kinase 4|renal carcinoma NY-REN-64
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRAK4, check out the IRAK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRAK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRAK4 Antibody Picoband® (PA1942)
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No publications found for PA1942
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Customer Q&As
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6 Customer Q&As for Anti-IRAK4 Antibody Picoband®
Question
My question regarding product PA1942, anti-IRAK4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Thomas
Verified customer
Asked: 2019-08-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1942 anti-IRAK4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-01
Question
I see that the anti-IRAK4 antibody PA1942 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-04
Question
Does PA1942 anti-IRAK4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Mangal
Verified customer
Asked: 2018-08-13
Answer
It shows on the product datasheet, PA1942 anti-IRAK4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-13
Question
I am interested in to test anti-IRAK4 antibody PA1942 on rat body of pancreas for research purposes, then I may be interested in using anti-IRAK4 antibody PA1942 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Jha
Verified customer
Asked: 2017-06-14
Answer
The products we sell, including anti-IRAK4 antibody PA1942, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-14
Question
We are currently using anti-IRAK4 antibody PA1942 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
E. Wu
Verified customer
Asked: 2016-08-17
Answer
The anti-IRAK4 antibody (PA1942) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for body of pancreas using anti-IRAK4 antibody PA1942. Let me know if you need anything else.
D. Anderson
Verified customer
Asked: 2014-05-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-05-28