|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-IRAK4 Antibody|
|Description||Rabbit IgG polyclonal antibody for Interleukin-1 receptor-associated kinase 4(IRAK4) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-IRAK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1942)|
|Replacement Item||This antibody may replace the following items: sc-134368|sc-34469|sc-34470|sc-374349|sc-99154 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK4(45-59aa DDRYNQFHIRRFEAL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Interleukin-1 receptor-associated kinase 4|
|Molecular Weight||51530 MW|
|Protein Function||Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino- mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA- IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.|
|Subcellular Localization||Cytoplasm .|
|Alternative Names||Interleukin-1 receptor-associated kinase 4;IRAK-4;126.96.36.199;Renal carcinoma antigen NY-REN-64;IRAK4;|
|Research Areas|||immunology|innate immunity|cytokines|interleukins| signal transduction|protein phosphorylation|ser / thr kinases|other kinases| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators| immunology|tlr signaling||
Background for Interleukin-1 receptor-associated kinase 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-IRAK4 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-IRAK4 Antibody Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Lane 3: MCF-7 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,