Anti-IRF1 Rabbit Monoclonal Antibody

IRF1 antibody

Boster Bio Anti-IRF1 Rabbit Monoclonal Antibody catalog # M00580. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00580
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-IRF1 Rabbit Monoclonal Antibody

View all IRF1 Antibodies

SKU/Catalog Number

M00580

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-IRF1 Rabbit Monoclonal Antibody catalog # M00580. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IRF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00580)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DHE-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human IRF1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00580 is reactive to IRF1 in Human, Mouse, Rat

Applications

M00580 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

36.502kDa

Background of IRF1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For IRF1 (Source: Uniprot.org, NCBI)

Gene Name

IRF1

Full Name

Interferon regulatory factor 1

Weight

36.502kDa

Superfamily

IRF family

Alternative Names

interferon regulatory factor 1; IRF1; MAR; MARIRF-1 IRF1 IRF-1, MAR interferon regulatory factor 1 interferon regulatory factor 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IRF1, check out the IRF1 Infographic

IRF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IRF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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17 Customer Q&As for Anti-IRF1 Rabbit Monoclonal Antibody

Question

Our team were happy with the WB result of your anti-IRF1 Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta nucleus. cytoplasm. note=myd88-associated using this antibody. Is that expected? Could you tell me where is IRF1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

Based on literature, placenta does express IRF1. Generally IRF1 expresses in nucleus. cytoplasm. note=myd88-associated. Regarding which tissues have IRF1 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 1382447

Boster Scientific Support

Answered: 2020-04-16

Question

Is this M00580 anti-IRF1 Rabbit Monoclonal antibody reactive to the isotypes of IRF1?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

The immunogen of M00580 anti-IRF1 Rabbit Monoclonal antibody is A synthesized peptide derived from human IRF1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-26

Question

I have attached the WB image, lot number and protocol we used for blood using anti-IRF1 Rabbit Monoclonal antibody M00580. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-18

Question

I have a question about product M00580, anti-IRF1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00580 anti-IRF1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-23

Question

We are currently using anti-IRF1 Rabbit Monoclonal antibody M00580 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The anti-IRF1 Rabbit Monoclonal antibody (M00580) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-16

Question

I see that the anti-IRF1 Rabbit Monoclonal antibody M00580 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-09

Question

I am interested in to test anti-IRF1 Rabbit Monoclonal antibody M00580 on mouse blood for research purposes, then I may be interested in using anti-IRF1 Rabbit Monoclonal antibody M00580 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

The products we sell, including anti-IRF1 Rabbit Monoclonal antibody M00580, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-15

Question

We have seen staining in mouse pancreas. Any tips? Is anti-IRF1 Rabbit Monoclonal antibody supposed to stain pancreas positively?

H. Thomas

Verified customer

Asked: 2019-07-12

Answer

According to literature pancreas does express IRF1. According to Uniprot.org, IRF1 is expressed in blood, placenta, pancreas, among other tissues. Regarding which tissues have IRF1 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 1382447

Boster Scientific Support

Answered: 2019-07-12

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IRF1 Rabbit Monoclonal antibody M00580. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-07

Question

Would anti-IRF1 Rabbit Monoclonal antibody M00580 work on goat IP with pancreas?

Verified Customer

Verified customer

Asked: 2019-05-20

Answer

Our lab technicians have not validated anti-IRF1 Rabbit Monoclonal antibody M00580 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IRF1 Rabbit Monoclonal antibody M00580 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat pancreas in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-20

Question

Does anti-IRF1 Rabbit Monoclonal antibody M00580 work for IF with blood?

Verified Customer

Verified customer

Asked: 2019-03-26

Answer

According to the expression profile of blood, IRF1 is highly expressed in blood. So, it is likely that anti-IRF1 Rabbit Monoclonal antibody M00580 will work for IF with blood.

Boster Scientific Support

Answered: 2019-03-26

Question

We have tried in the past anti-IRF1 Rabbit Monoclonal antibody for WB on pancreas a few years ago. I am using mouse, and I plan to use the antibody for IP next. We want examining pancreas as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

J. Wu

Verified customer

Asked: 2018-12-25

Answer

I looked at the website and datasheets of our anti-IRF1 Rabbit Monoclonal antibody and it seems that M00580 has been validated on mouse in both WB and IP. Thus M00580 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-12-25

Question

I was wanting to use your anti-IRF1 Rabbit Monoclonal antibody for IF for mouse blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse blood identification?

Verified Customer

Verified customer

Asked: 2018-09-10

Answer

It shows on the product datasheet, M00580 anti-IRF1 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-10

Question

Is a blocking peptide available for product anti-IRF1 Rabbit Monoclonal antibody (M00580)?

Verified Customer

Verified customer

Asked: 2017-07-21

Answer

We do provide the blocking peptide for product anti-IRF1 Rabbit Monoclonal antibody (M00580). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-07-21

Question

Do you have a BSA free version of anti-IRF1 Rabbit Monoclonal antibody M00580 available?

K. Jones

Verified customer

Asked: 2017-04-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IRF1 Rabbit Monoclonal antibody M00580 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-04-24

Question

Will M00580 anti-IRF1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Dhar

Verified customer

Asked: 2015-12-14

Answer

As indicated on the product datasheet, M00580 anti-IRF1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-12-14

Question

I was wanting to use using your anti-IRF1 Rabbit Monoclonal antibody for immune system process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

R. Wu

Verified customer

Asked: 2013-06-27

Answer

We appreciate your inquiry. This M00580 anti-IRF1 Rabbit Monoclonal antibody is validated on rat kidney, jurkat cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-06-27

Order DetailsPrice
M00580

100μL

$370
M00580-30ul

30μL sample

$99
M00580-carrier-free

Carrier Free

$370

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