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Product Info Summary
| SKU: | M00580 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRF1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00580
BM4346 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRF1 Rabbit Monoclonal Antibody catalog # M00580. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00580)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DHE-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRF1
Reactive Species
M00580 is reactive to IRF1 in Human, Mouse, Rat
Observed Molecular Weight
48 kDa
Calculated molecular weight
36.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00580 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50
Positive Control
IHC: rat kidney tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded rat kidney, using IRF1 Antibody.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Hela cells, using IRF1 Antibody .
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Western blot analysis of IRF1 expression in Jurkat cell lysate.
Specific Publications For Anti-IRF1 Rabbit Monoclonal Antibody (M00580)
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-IRF1 Rabbit Monoclonal Antibody
Question
Our team were happy with the WB result of your anti-IRF1 Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta nucleus. cytoplasm. note=myd88-associated using this antibody. Is that expected? Could you tell me where is IRF1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Based on literature, placenta does express IRF1. Generally IRF1 expresses in nucleus. cytoplasm. note=myd88-associated. Regarding which tissues have IRF1 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 1382447
Boster Scientific Support
Answered: 2020-04-16
Question
Is this M00580 anti-IRF1 Rabbit Monoclonal antibody reactive to the isotypes of IRF1?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
The immunogen of M00580 anti-IRF1 Rabbit Monoclonal antibody is A synthesized peptide derived from human IRF1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-26
Question
I have attached the WB image, lot number and protocol we used for blood using anti-IRF1 Rabbit Monoclonal antibody M00580. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
I have a question about product M00580, anti-IRF1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00580 anti-IRF1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-IRF1 Rabbit Monoclonal antibody M00580 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-IRF1 Rabbit Monoclonal antibody (M00580) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
I see that the anti-IRF1 Rabbit Monoclonal antibody M00580 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-09
Question
I am interested in to test anti-IRF1 Rabbit Monoclonal antibody M00580 on mouse blood for research purposes, then I may be interested in using anti-IRF1 Rabbit Monoclonal antibody M00580 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The products we sell, including anti-IRF1 Rabbit Monoclonal antibody M00580, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-15
Question
We have seen staining in mouse pancreas. Any tips? Is anti-IRF1 Rabbit Monoclonal antibody supposed to stain pancreas positively?
H. Thomas
Verified customer
Asked: 2019-07-12
Answer
According to literature pancreas does express IRF1. According to Uniprot.org, IRF1 is expressed in blood, placenta, pancreas, among other tissues. Regarding which tissues have IRF1 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 1382447
Boster Scientific Support
Answered: 2019-07-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IRF1 Rabbit Monoclonal antibody M00580. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-07
Question
Would anti-IRF1 Rabbit Monoclonal antibody M00580 work on goat IP with pancreas?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
Our lab technicians have not validated anti-IRF1 Rabbit Monoclonal antibody M00580 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IRF1 Rabbit Monoclonal antibody M00580 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat pancreas in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
Does anti-IRF1 Rabbit Monoclonal antibody M00580 work for IF with blood?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
According to the expression profile of blood, IRF1 is highly expressed in blood. So, it is likely that anti-IRF1 Rabbit Monoclonal antibody M00580 will work for IF with blood.
Boster Scientific Support
Answered: 2019-03-26
Question
We have tried in the past anti-IRF1 Rabbit Monoclonal antibody for WB on pancreas a few years ago. I am using mouse, and I plan to use the antibody for IP next. We want examining pancreas as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
J. Wu
Verified customer
Asked: 2018-12-25
Answer
I looked at the website and datasheets of our anti-IRF1 Rabbit Monoclonal antibody and it seems that M00580 has been validated on mouse in both WB and IP. Thus M00580 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-25
Question
I was wanting to use your anti-IRF1 Rabbit Monoclonal antibody for IF for mouse blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
It shows on the product datasheet, M00580 anti-IRF1 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-10
Question
Is a blocking peptide available for product anti-IRF1 Rabbit Monoclonal antibody (M00580)?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
We do provide the blocking peptide for product anti-IRF1 Rabbit Monoclonal antibody (M00580). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-21
Question
Do you have a BSA free version of anti-IRF1 Rabbit Monoclonal antibody M00580 available?
K. Jones
Verified customer
Asked: 2017-04-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IRF1 Rabbit Monoclonal antibody M00580 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-04-24
Question
Will M00580 anti-IRF1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Dhar
Verified customer
Asked: 2015-12-14
Answer
As indicated on the product datasheet, M00580 anti-IRF1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-12-14
Question
I was wanting to use using your anti-IRF1 Rabbit Monoclonal antibody for immune system process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
R. Wu
Verified customer
Asked: 2013-06-27
Answer
We appreciate your inquiry. This M00580 anti-IRF1 Rabbit Monoclonal antibody is validated on rat kidney, jurkat cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-06-27
