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Product Info Summary
| SKU: | M00036-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IP, IHC-P, WB |
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Product info
Product Name
Anti-STAT1 Purified Monoclonal Antibody
SKU/Catalog Number
M00036-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-STAT1 Purified Monoclonal Antibody (Catalog# M00036-3). Tested in IP, WB, IHC-P application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-STAT1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00036-3)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SM1
Isotype
Mouse IgG2b
Immunogen
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE). The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Reactive Species
M00036-3 is reactive to STAT1 in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
87.3 kDa
Background of STAT1
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00036-3 is guaranteed for IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 5 μg/ml; positive tissue: lung macrophages.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Detection of Human phosphorylated STAT1[p Ser727] and total STAT1 by Western Blot. Western blot shows lysate of human malignant melanoma cells (short-term culture derived from a patient) in response to interferon treatment to induce phosphorylation of STAT1 at Ser727. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Specific band was detected for phosphorylated STAT1[Ser727] by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).
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Western blotting analysis of human STAT1 using mouse monoclonal antibody SM1 on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-STAT1 followed by IRDye800-conjugated anti-mouse secondary antibody. STAT1 was detected at approximately 90 kDa.
Specific Publications For Anti-STAT1 Purified Monoclonal Antibody (M00036-3)
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