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Product Info Summary
| SKU: | M02969-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Islet-1 ISL1 Monoclonal Antibody
SKU/Catalog Number
M02969-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Islet-1 ISL1 Monoclonal Antibody catalog # M02969-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Islet-1 ISL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02969-2)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1H9
Isotype
IgG
Immunogen
Purified recombinant fragment of human Islet-1 expressed in E. Coli.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02969-2 is reactive to ISL1 in Human
Calculated molecular weight
39.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02969-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blotting validation for Anti-Islet-1 ISL1 Monoclonal Antibody M02969-2
Western Blot (WB) analysis using Islet-1 Monoclonal Antibody against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Immunohistochemistry validation of ISL1 using Anti-Islet-1 ISL1 Monoclonal Antibody (M02969-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right)
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Immunofluorescent staining data of ISL1 using Anti-Islet-1 ISL1 Monoclonal Antibody (M02969-2).
Confocal Immunofluorescence (IF) analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using Islet-1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.Specific Publications For Anti-Islet-1 ISL1 Monoclonal Antibody (M02969-2)
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