Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband®

Western blot analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA2B antigen affinity purified polyclonal antibody (Catalog # PB9647) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA2B at approximately 120-135 kDa. The expected band size for ITGA2B is at 113 kDa.

The effects of rhTyrRS(Y341A) on megakaryocytes were studied by immunohistochemistry and ( H & E ) staining in the bone marrow and lung. ( H & E ) staining showed there was no increase in the number of bone marrow megakaryocyte cells in the ( B ) rhTyrRS (Y341A) (100 μg/kg) group (22.0 ± 1.2) or ( A ) PBS group (21 ± 1.25). However, the number of bone marrow megakaryocyte cells increased significantly in ( C ) the TPO group (33.5 ± 1.0) (***p < 0.001). Immunohistochemistry of bone marrow megakaryocytes (CD41 + ) also showed there was no increase in the number of bone marrow megakaryocyte cells in the ( E ) rhTyrRS (Y341A) (100 μg/kg) (23.3 ± 0.8) or ( D ) PBS group (22.3 ± 0 0.67), while the number of bone marrow megakaryocyte cells increased significantly in the ( F ) TPO group (35.2 ± 0.9) (***p < 0.001), mean ± SEM. ( G ) Statistics on the number of bone marrow megakaryocytes in H&E staining and immunohistochemistry (***P < 0.001). Lung ( H & E ) staining showed there was no increase in the number of lung megakaryocyte cells in the ( H ) PBS group (3.67 ± 0.49), while the number of lung megakaryocyte cells increased significantly in the ( I ) rhTyrRS (Y341A) (100 μg/kg) (7.33 ± 0.56) and ( J ) TPO group (6.33 ± 0.84) (***p < 0.001,**p < 0.01). Immunohistochemistry of lung megakaryocytes (CD41 + ) also showed there was no increase in the number of lung megakaryocyte cells in the ( K ) PBS group (3.17 ± 0.48), while the number of lung megakaryocyte cells increased significantly in the ( L ) rhTyrRS (Y341A) (100 μg/kg) (6.5 ± 0.43) and ( M ) TPO group (6.3 ± 0.42) (***p < 0.001), mean ± SEM. ( N ) Statistics on the megakaryocyte numbers in lungs using H&E staining and immunohistochemistry (***P < 0.001, **p < 0.01).
Index in PubMed under a CC BY license. PMID: 28939876

IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
ITGA2B was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
ITGA2B was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
ITGA2B was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.