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Product Info Summary
| SKU: | A02598 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Tyrosine-protein kinase JAK3 JAK3 Antibody
SKU/Catalog Number
A02598
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Tyrosine-protein kinase JAK3 JAK3 Antibody catalog # A02598. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine-protein kinase JAK3 JAK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02598)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human JAK3. AA range:751-800
Reactive Species
A02598 is reactive to JAK3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
125.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02598 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of 293T cells using JAK3 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from Jurkat cells, using JAK3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, JAK3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using JAK3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of Hela cell. 1, JAK3 Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
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Immunofluorescence analysis of rat-kidney tissue. 1, JAK3 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-Tyrosine-protein kinase JAK3 JAK3 Antibody (A02598)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Tyrosine-protein kinase JAK3 JAK3 Antibody
Question
Is there a BSA free version of anti-JAK3 antibody A02598 available?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-JAK3 antibody A02598 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-10
Question
Does anti-JAK3 antibody A02598 work for WB with fetal brain?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
According to the expression profile of fetal brain, JAK3 is highly expressed in fetal brain. So, it is likely that anti-JAK3 antibody A02598 will work for WB with fetal brain.
Boster Scientific Support
Answered: 2019-04-01
Question
I see that the anti-JAK3 antibody A02598 works with WB, what is the protocol used to produce the result images on the product page?
C. Mangal
Verified customer
Asked: 2019-01-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-01-16
Question
We need to test anti-JAK3 antibody A02598 on mouse fetal brain for research purposes, then I may be interested in using anti-JAK3 antibody A02598 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
The products we sell, including anti-JAK3 antibody A02598, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-21
