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Product Info Summary
| SKU: | PB9510 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-STAT1 Antibody Picoband®
SKU/Catalog Number
PB9510
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STAT1 Antibody Picoband® catalog # PB9510. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9510)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human STAT1, different from the related mouse sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9510 is reactive to STAT1 in Human, Mouse, Rat
Observed Molecular Weight
83, 91 kDa
Calculated molecular weight
87.3 kDa
Background of STAT1
Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9510 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human SiHa whole cell, human A549 whole cell, rat lung tissue, mouse lung tissue
FCM: A549 cell
Validation Images & Assay Conditions
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Western blot analysis of STAT1 using anti-STAT1 antibody (PB9510).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # PB9510) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 83, 91 kDa. The expected band size for STAT1 is at 83, 87 kDa.
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Flow Cytometry analysis of A549 cells using anti-STAT1 antibody (PB9510).
Overlay histogram showing A549 cells stained with PB9510 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (PB9510, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-STAT1 Antibody Picoband® (PB9510)
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Customer Q&As
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5 Customer Q&As for Anti-STAT1 Antibody Picoband®
Question
I was wanting to use using your anti-STAT1 antibody for tumor necrosis factor-mediated signaling pathway studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
We appreciate your inquiry. This PB9510 anti-STAT1 antibody is tested on rat liver tissue, testis tissue, tissue lysate, brain tissue, human placenta tissue, sw620 whole cell lysate, mouse intestine tissue, mammary cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-07
Question
We are currently using anti-STAT1 antibody PB9510 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The anti-STAT1 antibody (PB9510) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-26
Question
We purchased anti-STAT1 antibody for WB on b-cell a few months ago. I am using mouse, and We want to use the antibody for IHC next. We need examining b-cell as well as placenta testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
I took a look at the website and datasheets of our anti-STAT1 antibody and I see that PB9510 has been validated on mouse in both WB and IHC. Thus PB9510 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-19
Question
We have observed staining in rat leukemic t-cell. What should we do? Is anti-STAT1 antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2018-06-21
Answer
From what I have seen in literature leukemic t-cell does express STAT1. From what I have seen in Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-06-21
Question
My colleagues were satisfied with the WB result of your anti-STAT1 antibody. However we have observed positive staining in placenta testis cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
From what I have seen in literature, placenta testis does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-04-05
