Product Info Summary
| SKU: | A03690-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-JMJD6 Antibody
SKU/Catalog Number
A03690-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-JMJD6 Antibody (Catalog # A03690-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
JMJD6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-JMJD6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03690-1)
Host
Rabbit
Contents
JMJD6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
JMJD6 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human JMJD6. The immunogen is located within amino acids 70 - 120 of JMJD6.
Reactive Species
A03690-1 is reactive to JMJD6 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.5 kDa
Background of JMJD6
The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. JMJD6 was initially identified as a membrane protein that participates in phagocytosis but recent studies have shown that it has other functions when it is expressed in the cytosol and nucleus. JMJD6 is thought to play important roles in regulation of development and differentiation as knockdown experiments in mice resulted in neonatal lethality with severe defects in the morphology of numerous organs. JMJD6 also can catalyze the lysyl-hydroxylation of U2AF65, a protein involved with RNA splicing, suggesting that some of the functions attributed to JMJD6 may be due to its regulatory activity of RNA splicing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03690-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
JMJD6 antibody can be used for detection of JMJD6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of JMJD6 in human brain tissue lysate with JMJD6 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of JMJD6 in rat brain tissue with JMJD6 antibody at 20 μg/mL.
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Immunohistochemistry of JMJD6 in rat brain tissue with JMJD6 antibody at 2.5 μg/mL.
Specific Publications For Anti-JMJD6 Antibody (A03690-1)
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