Anti-Kallikrein 11/KLK11 Antibody

Rabbit Polyclonal Kallikrein 11/KLK11 Antibody. Validated in IHC, WB and tested in Human.

SKU PA1631
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Kallikrein 11/KLK11 Antibody
See all KLK11 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1631
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal Kallikrein 11/KLK11 Antibody. Validated in IHC, WB and tested in Human. Size: 100μg/vial.
Cite This Product Anti-Kallikrein 11/KLK11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1631)
Specificity Anti-Kallikrein 11/KLK11 Antibody (PA1631) reacts with Human KLK11, in native form and recombinant. Superfamily members of KLK11 are not reactive to PA1631.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-Kallikrein 11/KLK11 Antibody (PA1631) was raised against A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 11(233-250aa YTKVCKYVDWIQETMKNN)..
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-Kallikrein 11/KLK11 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Kallikrein 11/KLK11 Antibody (PA1631).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/antibody/pa1631 1 WB anti klk11 kallikrein 11 antibody.jpg

Anti-Kallikrein 11 antibody, PA1631, Western blotting
All lanes: Anti Kallikrein 11 (PA1631) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 31KD

/antibody/pa1631 2 IHC anti klk11 kallikrein 11 antibody.jpg

Anti-Kallikrein 11 antibody, PA1631, IHC(P)
IHC(P): Human Prostatic Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q9UBX7
Gene Name KLK11
Protein Name Kallikrein-11
Tissue Specificity Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). .
Alternative Names
  • Hippostasin antibody
  • hK11 antibody
  • Kallikrein 11 antibody
  • Kallikrein 11 isoform 1 preproprotein antibody
  • Kallikrein 11 isoform 2 precursor antibody
  • Kallikrein 11 precursor antibody
  • Kallikrein related peptidase 11 antibody
  • Kallikrein-11 inactive chain 2 antibody
  • KLK 11 antibody
  • Klk11 antibody
  • KLK11 antibody
  • MGC33060 antibody
  • Protease serine 20 trypsin like antibody
  • Protease serine trypsin like antibody
  • PRSS20 antibody
  • Serine protease 20 antibody
  • TLSP antibody
  • Trypsin like protease antibody
  • Trypsin-like protease antibody
Show more
Subcellular Localization Isoform 1: Secreted.
Molecular Weight 31059 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. .
Research Areas Cancer, Cell Biology, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis / Ubiquitin, Proteolytic Enzymes, Signal Transduction

*You can search these to find other products in these research areas.
Background KLK11(KALLIKREIN 11), also called PRSS20 or TLSP, is a protein that in humans is encoded by the KLK11 gene. KLK11 is a member of the kallikrein subfamily of serine proteases, which are involved in a variety of enzymatic processes. The KLK11 gene is mapped to 19q13.41. The KLK11 gene contains 6 exons, the first of which is noncoding. KLK11 shares 48% amino acid sequence identity with mouse neuropsin, 43% identity with both human trypsin-1 and human kallikrein, and 38% identity with the mouse nerve growth factor gamma subunit. Alternate splicing of the KLK11 gene results in two transcript variants encoding two different isoforms which are differentially expressed. Western blot analysis of recombinant KLK11 suggested that the protein is secreted and posttranslationally processed.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-Kallikrein 11/KLK11 Antibody (PA1631)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1631)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships next business day.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Publications

Comparative Transcriptome Analysis of Fetal Skin Reveals Key Genes Related to Hair Follicle Morphogenesis in Cashmere Goats

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.