Human KLK11/Kallikrein-11 ELISA Kit PicoKine®

Kallikrein 11 ELISA kit for Human

Human KLK11/Kallikrein 11 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KLK11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1166
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human KLK11/Kallikrein-11 ELISA Kit PicoKine®

View all Kallikrein 11 ELISA kits

SKU/Catalog Number

EK1166

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human KLK11/Kallikrein 11 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KLK11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human KLK11/Kallikrein-11 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1166)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-N250

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1166 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1166 is reactive to KLK11 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1166 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Kallikrein 11

Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Kit Components

Catalog Number Description Quantity
EK1166-CAP Anti-Human KLK11 Pre-coated 96-well strip microplate 1
EK1166-ST Human KLK11 Standard 2 vials, 10 ng/tube
EK1166-DA Human KLK11 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0250.1950.3010.5330.9421.3511.7772.025

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Kallikrein 11, Dilution ratio of 1:1, concentration in serum and plasma is 200-800 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human KLK11/Kallikrein-11 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)217105448882329944808
Standard deviation12.883.2624016.9392.44259.63
CV (%)4.9%7.9%4.9%7.3%9.3%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Kallikrein 11 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12172062332152179.734.4%
Sample 21054103111001045105725.862.4%
Sample 348984850451852914889274.245.6%
*number of samples for each test n=16.

Gene/Protein Information For KLK11 (Source: Uniprot.Org, NCBI)

Gene Name

KLK11

Full Name

Kallikrein-11

Weight

31.059kDa

Superfamily

peptidase S1 family

Alternative Names

EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Hippostasin; hK11; Kallikrein 11; kallikrein-related peptidase 11; KLK11; MGC33060; PRSS20kallikrein-11; Serine protease 20; TLSPhippostasin; Trypsin-like protease KLK11 PRSS20, TLSP kallikrein related peptidase 11 kallikrein-11|hK11|hippostasin|serine protease 20|trypsin-like protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KLK11, check out the KLK11 Infographic

KLK11 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KLK11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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11 Customer Q&As for Human KLK11/Kallikrein-11 ELISA Kit PicoKine®

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Kallikrein 11 in Human cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-09-22

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Kallikrein 11 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-09-22

Question

Q: how to thaw whole blood sample for Kallikrein 11 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-05-10

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Kallikrein 11 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-05-10

Question

Which proteins were tested to determine that there were no cross-reactivity with relevant proteins?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

The following recombinant proteins were tested and no significant cross-reactivity or interference was observed. <br> Recombinant human KLK1 <br> Recombinant human KLK3 <br> Recombinant human KLK5

Boster Scientific Support

Answered: 2020-03-05

Question

Is this kit able to distinguish between the active and inactive form?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

The kit is not able to distinguish the active and inactive form.

Boster Scientific Support

Answered: 2020-01-09

Question

Q: if the enzyme conjugated Kallikrein 11 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Kallikrein 11 antigen?

D. Chakrabarti

Verified customer

Asked: 2019-10-02

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-10-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-08-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-08-17

Question

Q: What is the optimal O.D. value for Kallikrein 11 ELISA kit? I used your Kallikrein 11 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Kallikrein 11 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Kallikrein 11 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Kallikrein 11 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-06-27

Question

How did you confirm it was KLK11? Have you distiguished or designed EK1166 to detect the active or inactive form?

Verified customer

Asked: 2019-05-09

Answer

The immunogen sequence of the capture antibody, detection antibody and the standard in the Human KLK11/Kallikrein 11 ELISA Kit PicoKine™ (EK1166) is E19-N250 of human KLK11. The kit doesn't cross react with other proteins and is specific to human KLK11. It is not able to distiguish the active and inactive form.

Boster Scientific Support

Answered: 2019-05-10

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-03-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-03-03

Question

Q: Can Kallikrein 11 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-05-18

Question

Q: how do I analyze ELISA data? I have performed Kallikrein 11 level in cell lysates.

Verified Customer

Verified customer

Asked: 2017-02-12

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-02-12

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