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KEAP1 antibody (A00514-3) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 16 Publications

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a00514 3 keap1 primary antibodies fcm testing 3

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Flow Cytometry analysis of U251 cells using anti-KEAP1 antibody (A00514-3).
Overlay histogram showing U251 cells stained with A00514-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEAP1 Antibody (A00514-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-KEAP1 Antibody Picoband®

KEAP1 antibody

16 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, ICC, WB

Tested Applications

KEAP1 antibody was positively detected by WB in:

human Hela whole cell, human Jurkat whole cell, human 293T whole cell, human HepG2 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

KEAP1 antibody was positively detected by ICC/IF in:

A431 cell

KEAP1 antibody was positively detected by FC in:

U251 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-KEAP1 Antibody Picoband®
View all KEAP1 Antibodies

Form

Lyophilized

Uniprot

Q14145

Gene ID

9817

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human KEAP1 recombinant protein (Position: K84-K312).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

66-72 kDa

Calculated Molecular Weight

69.7 kDa

Background of KEAP1

KEAP1 (KELCH-LIKE ECH-ASSOCIATED PROTEIN 1), is a protein that in humans is encoded by the Keap1 gene. The KIAA0132 gene is mapped on 19p13.2. Keap1 contains a central BTB/POZ domain and a C-terminal double glycine repeat (DGR), or Kelch, module. Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. In the presence of the electrophilic agent diethylmalate, Nrf2 activity is released from Keap1 and Nrf2 translocate to the nucleus. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which, in turn, facilitates the ubiquitination and subsequent proteolysis of Nrf2. Because Nrf2 activation leads to a coordinated antioxidant and anti-inflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target.

Cite This Product

Anti-KEAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00514-3)

Protocols

Western Blot protocol for KEAP1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for KEAP1 antibody

Review Protocol

Flow Cytometry (FC) protocol for KEAP1 antibody

Review Protocol

Publications

Nonpolysaccharide fraction of Lonicerae japonicae Flos attenuates cyclophosphamide-induced immunosuppression associated with modulation of the Keap1/Nrf2/HO-1/GPX4 signalling pathway

Published: 2026-07-17

Journal: Frontiers in Pharmacology

Application: WB

Impact Factor: 5.40

Species: Mouse

PMID: 42539646

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Anti-KEAP1 Antibody Picoband®

KEAP1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Nonpolysaccharide fraction of Lonicerae japonicae Flos attenuates cyclophosphamide-induced immunosuppression associated with modulation of the Keap1/Nrf2/HO-1/GPX4 signalling pathway

Yangjing Shugan decoction targets the Sirt1/Nrf2 antioxidant pathway and gut microbiota-metabolite axis to protect against premature ovarian failure

Silibinin Attenuates 3-Nitropropionic Acid-Induced Ovarian Toxicity by Alleviating Oxidative Stress and Granulosa Cell Apoptosis

Study on the effect of deer bone in improving rheumatoid arthritis based on the “drug-target-pathway” association network

Exo-miR-1290-induced by COX-2 overexpression promotes cancer-associated fibroblasts activation and tumor progression by CUL3-Nrf2 pathway in lung adenocarcinoma

Untargeted serum and liver metabolomics analyses reveal the gastroprotective effect of polysaccharide from Evodiae fructus on ethanol-induced gastric ulcer in mice

Pirfenidone attenuates nonalcoholic fatty liver disease through activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury

Tilapia Skin Peptides Ameliorate Cyclophosphamide-Induced Anxiety- and Depression-Like Behavior via Improving Oxidative Stress, Neuroinflammation, Neuron Apoptosis, and Neurogenesis in Mice

Senescence marker protein30 protects lens epithelial cells against oxidative damage by restoring mitochondrial function

Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway

Study of the selenizing Codonopsis pilosula polysaccharides protects RAW264.7 cells from hydrogen peroxide-induced injury

Andrographolide protects chondrocytes from oxidative stress injury by activation of the Keap1–Nrf2–Are signaling pathway

Astaxanthin Promotes Nrf2/ARE Signaling to Alleviate Renal Fibronectin and Collagen IV Accumulation in Diabetic Rats

Polysaccharide from Ostrea rivularis attenuates reproductive oxidative stress damage via activating Keap1-Nrf2/ARE pathway

Individual and combined antioxidant effects of ginsenoside F2 and cyanidin-3-O-glucoside in human embryonic kidney 293 cells

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