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Product Info Summary
| SKU: | PB9069 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GDNF Antibody Picoband®
SKU/Catalog Number
PB9069
PB0045 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GDNF Antibody Picoband® catalog # PB9069. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GDNF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9069)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GDNF recombinant protein (Position: S78-I211). Human GDNF shares 93% amino acid (aa) sequence identity with both mouse and rat GDNF.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9069 is reactive to GDNF in Human, Rat
Observed Molecular Weight
24 kDa
Calculated molecular weight
23.7 kDa
Background of GDNF
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF is also a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. In addition to its potential role in the differentiation and survival of central nervous system neurons, it has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9069 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of GDNF using anti-GDNF antibody (PB9069).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDNF antigen affinity purified polyclonal antibody (Catalog # PB9069) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDNF at approximately 24 kDa. The expected band size for GDNF is at 24 kDa.
Specific Publications For Anti-GDNF Antibody Picoband® (PB9069)
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Customer Q&As
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7 Customer Q&As for Anti-GDNF Antibody Picoband®
Question
My question regarding product PB9069, anti-GDNF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9069 anti-GDNF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-25
Question
Is PB9069 able to detect both preGDNF and mature GDNF?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
PB9069 is able to detect both preGDNF and mature GDNF.
Boster Scientific Support
Answered: 2019-08-16
Question
We are currently using anti-GDNF antibody PB9069 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
The anti-GDNF antibody (PB9069) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-22
Question
Is there a BSA free version of anti-GDNF antibody PB9069 available?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GDNF antibody PB9069 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal kidney using anti-GDNF antibody PB9069. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
Would anti-GDNF antibody PB9069 work on horse WB with quadriceps femoris?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
Our lab technicians have not tested anti-GDNF antibody PB9069 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GDNF antibody PB9069 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse quadriceps femoris in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-11
Question
I was wanting to use to test anti-GDNF antibody PB9069 on human fetal kidney for research purposes, then I may be interested in using anti-GDNF antibody PB9069 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Li
Verified customer
Asked: 2017-04-10
Answer
The products we sell, including anti-GDNF antibody PB9069, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-10
