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- Table of Contents
MKI67 antibody (A00254-1) performs well in ELISA, IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1).
Ki67/MKI67 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Ki67/MKI67 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Ki67/MKI67 Antibody
MKI67 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC |
Tested Applications
MKI67 antibody was positively detected by IHC in:
human colonic adenocarcinoma tissue, human lung cancer tissue, human renal carcinoma tissue, human tonsil tissue, human laryngeal squamous cell carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
ELISA
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Ki67/MKI67 Antibody
View all MKI67 Antibodies
View all MKI67 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Ki67/MKI67 recombinant protein (Position: R5-Q2358).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
358.7 kDa
Background of MKI67
Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Cite This Product
Anti-Ki67/MKI67 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00254-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
90%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for MKI67 antibody
Publications
Application:
Species:
A Novel HDAC6 Inhibitor Ameliorates Imiquimod-Induced Psoriasis-Like Inflammation in Mice
Published: 2025-07-31
Journal: MOLECULES
Application: IF
Impact Factor: 4.60
Species: Mouse
PMID: 40807399
Downregulation of GFPT2 enhances cisplatin chemotherapy sensitivity in STK11/KRAS mutant non-small cell lung cancer by regulating the hexosamine biosynthesis pathway, resisting tumor growth
Published: 2025-04-19
Journal: CYTOKINE
Application: IHC
Impact Factor: 3.70
Species: Mouse
PMID: 40253947
Methotrexate-modified docetaxel liposome targeting with ginsenoside Rh2 as a membrane stabilizer for the treatment of ovarian cancer
Published: 2024-06-27
Journal: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
Application: IHC,IF
Impact Factor: 4.50
Species: Mouse
PAFAH1B3 is a KLF9 target gene that promotes proliferation and metastasis in pancreatic cancer
Published: 2024-04-22
Journal: Scientific Reports
Application: IHC
Impact Factor: 3.80
Species: Human
PMID: 38649699
Network pharmacology-based investigation of the effects of Shenqi Fuzheng injection on glioma proliferation and migration via the SRC/PI3K/AKT signaling pathway
Published: 2024-03-30
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 4.80
Species: Mouse
PMID: 38561056
TIAM2 promotes proliferation and invasion of osteosarcoma cells by activating the JAK2/STAT3 signaling pathway
Published: 2022-11-14
Journal: Journal of Bone Oncology
Application: IHC
Impact Factor: 4.49
Species: Mouse
PMID: 36419799
Construction of Urokinase-Type Plasminogen Activator Receptor-Targeted Heterostructures for Efficient Photothermal Chemotherapy against Cervical Cancer To Achieve Simultaneous Anticancer and Antiangiogenesis
Published: 2019-10-07
Journal: ACS Applied Materials & Interfaces
Application: IHC
Impact Factor: 8.46
Species: Human
PMID: 31588724
Synergistic inhibitory effects of capsaicin combined with cisplatin on human osteosarcoma in culture and in xenografts
Published: 2018-10-16
Journal: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
Application: IHC
Impact Factor: 6.22
Species: Mouse
PMID: 30326933
KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1
Published: 2015-09-11
Journal: Oncotarget
Application: IHC
Impact Factor: 6.36
Species: Mouse
PMID: 26397136
FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in endometrial cancer
Published: 2014-02-11
Journal: BMC CANCER
Application: IHC
Impact Factor: 3.32
Species: Mouse
PMID: 24512546
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Product Promise
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Order Product
A00254-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026