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Product Info Summary
| SKU: | P00209-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00209-1
BM4328 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody catalog # P00209-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00209-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DFH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Cdk1/2 (T14)
Reactive Species
P00209-1 is reactive to CDK1 in Human, Mouse, Rat
Observed Molecular Weight
34 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00209-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human A431 whole cell, human Hacat whole cell, human SiHa whole cell, rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue
IHC: rat spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Cdk1/2 (T14) using anti-Phospho-Cdk1/2 (T14) antibody (P00209-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-Cdk1/2 (T14) antigen affinity purified monoclonal antibody (Catalog # P00209-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-Cdk1/2 (T14) at approximately 34 kDa. The expected band size for Phospho-Cdk1/2 (T14) is at 34 kDa.
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Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody.
Specific Publications For Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody (P00209-1)
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5 Customer Q&As for Anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal Antibody
Question
We have been able to see staining in mouse lymphoblast. What should we do? Is anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody supposed to stain lymphoblast positively?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
From what I have seen in literature lymphoblast does express CDK1. From what I have seen in Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody P00209-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody (P00209-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
We ordered your anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody for WB on cervix carcinoma erythroleukemia last year. I am using rat, and I plan to use the antibody for IP next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
I have checked the website and datasheets of our anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody and I see that P00209-1 has been tested on rat in both WB and IP. Thus P00209-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-18
Question
Our lab were well pleased with the WB result of your anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
From literature, cervix carcinoma does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-21
Question
My question regards using your anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody for tp53 regulates transcription of genes involved in g2 cell cycle arrest studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you for your inquiry. This P00209-1 anti-Phospho-Cdk1/2 (T14) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-29
