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- Table of Contents
PRC1 antibody (PB9814) performs well in Flow Cytometry, IP, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of 293T cells using anti-PRC1 antibody (PB9814).
Overlay histogram showing 293T cells stained with PB9814 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRC1 Antibody (PB9814, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing 293T cells stained with PB9814 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRC1 Antibody (PB9814, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PRC1 Antibody Picoband®
PRC1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, WB |
Tested Applications
PRC1 antibody was positively detected by WB in:
human U251 whole cell, human Hela whole cell, human 293T whole cell, human Hacat whole cell, rat testis tissue, rat liver tissue, mouse testis tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PRC1 antibody was positively detected by IP in:
293T cell
PRC1 antibody was positively detected by FC in:
293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Rat
Tested
Mouse
Tested
Monkey
Predicted
Dog
Predicted
Bovine
Predicted
Sheep
Predicted
Product Information
Product Name
Anti-PRC1 Antibody Picoband®
View all PRC1 Antibodies
View all PRC1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRC1 recombinant protein (Position: M1-E233). Human PRC1 shares 85% amino acid (aa) sequence identity with mouse PRC1.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated Molecular Weight
71.6 kDa
Background of PRC1
Protein Regulator of cytokinesis 1 (PRC1) is a protein that in humans is encoded by the PRC1 gene and is involved in cytokinesis. This gene is mapped to chromosome 15q26.1 based on an alignment of the PRC1 sequence with the genomic sequence. PRC1 protein is expressed at relatively high levels during S and G2/M phases of the cell cycle before dropping dramatically after mitotic exit and entrance into G1 phase. It is located in the nucleus during interphase, becomes associated with the mitotic spindle in a highly dynamic manner during anaphase, and localizes to the cell midbody during cytokinesis. And this protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-PRC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9814)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
97%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Rabbit
87%
Moderate Homology
Mouse
85%
Moderate Homology
Rat
84%
Moderate Homology
Zebrafish
60%
Low homology
Drosophila
24%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PRC1 antibody
Immunoprecipitation (IP) protocol for PRC1 antibody
Flow Cytometry (FC) protocol for PRC1 antibody
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Product Promise
We promise Anti-PRC1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9814
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026