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Anti-PRC1 Antibody Picoband™
Boster Bio Anti-PRC1 Antibody Picoband™ catalog # PB9814. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9814)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human PRC1 recombinant protein (Position: M1-E233). Human PRC1 shares 85% amino acid (aa) sequence identity with mouse PRC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
PB9814 is reactive to PRC1 in Human
PB9814 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRC1 using anti-PRC1 antibody (PB9814).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: MCF-7 Whole Cell Lysate,
Lane 2: SGC Whole Cell Lysate,
Lane 3: HELA Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 antigen affinity purified polyclonal antibody (Catalog # PB9814) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRC1 at approximately 75 kDa, 80 kDa. The expected band size for PRC1 is at 75 kDa, 80 kDa.
Gene/Protein Information For PRC1 (Source: Uniprot.Org, NCBI)
Protein regulator of cytokinesis 1
anaphase spindle elongation 1 homolog; ASE1; MGC1671; MGC3669; protein regulating cytokinesis 1; protein regulator of cytokinesis 1 PRC1 ASE1 protein regulator of cytokinesis 1 protein regulator of cytokinesis 1|anaphase spindle elongation 1 homolog|protein regulating cytokinesis 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PRC1, check out the PRC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PRC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB9814
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