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Mki67 antibody (A00254) performs well in ELISA, IHC and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 68 Publications

a00254 ki67 primary antibodies ihc testing 1_1

IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Ki67/Mki67 Antibody

Mki67 antibody

68 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, IHC

Tested Applications

Mki67 antibody was positively detected by IHC in:

human tonsil tissue, human lung cancer tissue, human thyroid cancer tissue, mouse thymus tissue, rat thymus tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Mouse

Tested

Recommend

Rat

Tested

Recommend

Product Information

Product Name

Anti-Ki67/Mki67 Antibody
View all MKI67 Antibodies

Form

Lyophilized

Uniprot

E9PVX6

Gene ID

17345

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Mki67 recombinant protein (Position: F29-S3177).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Calculated Molecular Weight

350.9 kDa

Background of Mki67

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Cite This Product

Anti-Ki67/Mki67 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00254)

Protocols

Immunohistochemistry (IHC) protocol for Mki67 antibody

Review Protocol

Publications

Iron homeostasis–driven Fe2+-responsive multimodal probe for NIR-II imaging-guided photothermal therapy of pancreatic cancer

Published: 2026-07-27

Journal: CHEMICAL ENGINEERING JOURNAL

Application: IF

Impact Factor: 12.50

Species: Mouse

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Free Secondary Antibody

Anti-Ki67/Mki67 Antibody

Mki67 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Iron homeostasis–driven Fe2+-responsive multimodal probe for NIR-II imaging-guided photothermal therapy of pancreatic cancer

CBLC-mediated ubiquitination stabilizes METTL1, enhances N7-methylguanosine modification of ESRRA, and promotes the progression of endometrial cancer

Non-classic deubiquitinase USP13 inhibits bladder cancer metastasis through destabilizing cytoplasmic KDM3A

Peroxidasin promotes malignant progression by enhancing glycolytic metabolism in glioblastoma through the regulation of LDHA

Annexin A1 enhances liver repair after acetaminophen-induced liver injury by regulating neutrophils function

Microbial diagnostic features identified across populations possess potential antitumor properties in breast cancer

Non-classic deubiquitinase USP13 inhibits bladder cancer metastasis through destabilizing cytoplasmic KDM3A

IER3 Facilitates Tumor Progression and Aberrant Glycolysis via Activating wnt/β-Catenin Pathway in Oral Squamous Cell Carcinoma

Inhibition of Ovarian Cancer Growth, Metastasis and Reverse the Tumor Microenvironment by Dual Drug-Loaded Polymer Micelle Targeting Tumor Microenvironment

Fluorescent Nanoparticles Achieve Efficient Photothermal Conversion and Enhanced Antitumor Efficacy Through Intermolecular Aggregation-Caused Quenching

Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

Methotrexate-modified docetaxel liposome targeting with ginsenoside Rh2 as a membrane stabilizer for the treatment of ovarian cancer

Cardamomin Inhibits the Proliferation and Tumorigenesis of Bladder Cancer by ESR1 in PI3K/AKT Pathway

Engineering of phytic acid-coated Prussian nanoparticles for combined Nitric oxide and Low-Temperature photothermal therapy of osteosarcoma

PAFAH1B3 is a KLF9 target gene that promotes proliferation and metastasis in pancreatic cancer

5S-Heudelotinone alleviates experimental colitis by shaping the immune system and enhancing the intestinal barrier in a gut microbiota-dependent manner

Berberrubine is a novel and selective IMPDH2 inhibitor that impairs the growth of colorectal cancer

Integrating network pharmacology and bioinformatics to explore and experimentally verify the regulatory effect of Buyang Huanwu decoction on glycolysis and angiogenesis after cerebral infarction

Transcriptomics and Molecular Docking Reveal the Potential Mechanism of Lycorine Against Pancreatic Cancer

PSME3 induces radioresistance and enhances aerobic glycolysis in cervical cancer by regulating PARP1

CircNCOR1 regulates breast cancer radiotherapy efficacy by regulating CDK2 via hsa-miR-638 binding

Upregulation of long intergenic non-coding RNA LINC00326 inhibits non-small cell lung carcinoma progression by blocking Wnt/β-catenin pathway through modulating the miR-657/dickkopf WNT signaling pathway inhibitor 2 axis

3D bioprinting of heterogeneous tissue-engineered skin containing human dermal fibroblasts and keratinocytes

MicroRNA-376b-3p Suppresses Choroidal Neovascularization by Regulating Glutaminolysis in Endothelial Cells

E2F1- deficient adipose-derived stem cells improve skin wound closure in mice by upregulating paracrine expression of VEGF and TGF-β1

Aloperine executes antitumor effects through the induction of apoptosis and cell cycle arrest in prostate cancer in vitro and in vivo

Dual Inhibition of CDK12/CDK13 Targets Both Tumor and Immune Cells in Ovarian Cancer

Niobium Diselenide Nanosheets: An Emerging Biodegradable Nanoplatform for Efficient Cancer Phototheranostics in the NIR-II Window

Intravoxel Incoherent Motion Diffusion-Weighted MR Imaging for Monitoring the Immune Response of Immunogenic Chemotherapy

Magnolol alleviates hypoxia-induced pulmonary vascular remodeling through inhibition of phenotypic transformation in pulmonary arterial smooth muscle cells

Hsa_circ_0076931 suppresses malignant biological properties, down-regulates miR-6760-3p through direct binding, and up-regulates CCBE1 in glioma

Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter

Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells

Simultaneous Inhibition of Ornithine Decarboxylase 1 and Pyruvate Kinase M2 Exerts Synergistic Effects Against Hepatocellular Carcinoma Cells

RIP1 promotes proliferation through G2/M checkpoint progression and mediates cisplatin-induced apoptosis and necroptosis in human ovarian cancer cells

Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer-associated fibroblasts of TSCC

Knockdown of YAP inhibits growth in Hep-2 laryngeal cancer cells via epithelial-mesenchymal transition and the Wnt/β-catenin pathway

Saikosaponin A Inhibits Breast Cancer by Regulating Th1/Th2 Balance

MicroRNA-125a inhibits tumorigenesis by targeting Smurf1 in colorectal carcinoma

Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis

MicroRNA-200a suppresses prostate cancer progression through BRD4/AR signaling pathway

Circular RNA EIF6 (Hsa_circ_0060060) sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation

Synergistic inhibitory effects of capsaicin combined with cisplatin on human osteosarcoma in culture and in xenografts

Identification and differentiation therapy strategy of pterygium in vitro

MiR-199a-5p Inhibits the Growth and Metastasis of Colorectal Cancer Cells by Targeting ROCK1

Involvement of aberrantly activated HOTAIR/EZH2/miR-193a feedback loop in progression of prostate cancer

Downregulation of Survivin Gene Expression Affects Ionizing Radiation Resistance of Human T98 Glioma Cells

The mTOR inhibition in concurrence with ERK1/2 activation is involved in excessive autophagy induced by glycyrrhizin in hepatocellular carcinoma

Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway

WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects

MicroRNA-744 promotes prostate cancer progression through aberrantly activating Wnt/β-catenin signaling

Cell proliferation and differentiation during the three dimensional reconstitution of eccrine sweat glands

Core-shell nanocarriers with high paclitaxel loading for passive and active targeting

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

Quercetin inhibits angiogenesis by targeting calcineurin in the xenograft model of human breast cancer

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo

MicroRNA-150 suppresses cell proliferation and metastasis in hepatocellular carcinoma by inhibiting the GAB1-ERK axis

Leukemia inhibitory factor promotes tumor growth and metastasis in human osteosarcoma via activating STAT3

MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis

Galactosylated poly(ethylene glycol) methacrylate-st-3-guanidinopropyl methacrylamide copolymers as siRNA carriers for inhibiting Survivin expression in vitro and in vivo

A novel protein extracted from foxtail millet bran displays anti-carcinogenic effects in human colon cancer cells

Targeting of Histone Deacetylases to Reactivate Tumour Suppressor Genes and Its Therapeutic Potential in a Human Cervical Cancer Xenograft Model

Interleukin-15 gene therapy and the mammalian target of rapamycin inhibitor everolimus inhibit the growth of metastatic breast cancer

Effects of co-culturing BMSCs and auricular chondrocytes on the elastic modulus and hypertrophy of tissue engineered cartilage

Roux-en-Y Gastric Bypass-Induced Improvement of Glucose Tolerance and Insulin Resistance in Type 2 Diabetic Rats Are Mediated by Glucagon-Like Peptide-1

Low-level expression of let-7a in gastric cancer and its involvement in tumorigenesis by targeting RAB40C