Anti-Ki67/Mki67 Antibody

IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

miR-150 suppresses tumor growth and metastasis in vivo . A. Transduced MHCC97-H cells were injected subcutaneously into nude mice (n=5). After 28 days, the mice were euthanized, and the tumors were excised. Representative tumors are shown. B. Growth curve of tumor volumes. C. Tumor weight. D. Representative H&E and immunohistochemical staining for Ki-67, GAB1 and phospho-ERK1/2 in tumors are shown. E. Representative bioluminescence images 35 days after injection are shown. F. The luminescence intensity of lung metastases from MHCC97-H-luc-miR-150 cells was weaker than that of lung metastases from MHCC97-H-luc-miR-NC cells. G. miR-150 reduced the incidence of lung metastases. H. miR-150 overexpression significantly reduced the number of lung metastatic nodules. I. Representative H&E and immunohistochemical staining for Ki-67, GAB1 and phospho-ERK1/2 in lung metastatic nodules are shown. * P <0.05.
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Kaplan-Meier analysis of overall survival in HCC patients according to Nek7 and Ki-67 expression. A. Higher Nek7 expression was closely correlated with poor overall survival ( p < 0.001). B. The HCC group with Ki-67 higher expression indicated poorer overall survival ( p < 0.001).
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Nek7 gene silencing suppressed growth of SMMC7721 xenograft tumors. A. Tumors isolated from mice of different groups are shown. B. Growth curves of xenograft tumors from the experiments with BALB/c nude mice. Changes in tumor volumes measured at the indicated days are shown. C. Therapeutic lenti-shNek7-1 reduced the tumor weight. Real-time PCR and Western blot assays to determine the mRNA and protein levels of cell growth associated genes, Bcl-2, cyclinD1 and cyclinB1 in HCC cells D. and dissected tumors E, F. Immunohistochemical staining was used to detect the Nek7 and Ki-67 expression pattern in xenograft tumors from different groups. β-actin was used as internal control for Western blot and Real-time PCR. Experiments were repeated three times. (* p < 0.05).
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Immunohistochemistry of Nek7 and Ki-67 on HCC specimens. Representative immunohistochemical staining of HCC specimens with different histologic grade, as determined using anti-Nek7 and anti-Ki-67 antibodies. A. The expression status of Nek7 in low-grade HCC tissue. B. An example of moderate-differentiated HCC with Nek7 expression. C. Nek7 expression in high-grade HCC tissue. D. Ki-67 expression status in low-grade HCC tissue. E. Example of moderate-differentiated HCC with Ki-67 expression. F. Ki-67 expression in high-grade HCC tissue.
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Immunohistochemistry staining for YAP, KI-67 and Bcl-2 protein in xenograft tumour model and pulmonary metastasis model. Cytoplasmic staining was considered to be positive for YAP, KI-67 and Bcl-2. b & f Higher YAP expression in both xenograft tumour and pulmonary models. c & d High YAP expression mainly in the nucleus of LSCC. e & f Low YAP expression in LSCC in both the cytoplasm and nucleus of tumour cells. g & h Low expression of YAP protein mainly in the nucleus of LSCC. [A, a, E, e] × 40; [B, b, C, c, D, d, F, f, G, g, H, h] × 100
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LINC00326 downregulation accelerates tumor growth in vivo. a Appearance of tumors four weeks after injection, showing the blank control, negative control, LINC00326 overexpression, shCTRL, and LINC00326 knockdown. b Tumor volumes in the five groups (mean ± SD) at time of sacrifice. c , d LINC00326 and miR-657 mRNA expression in tumors from blank, OE-CTRL, OE- LINC00326, shCTRL, and shLINC00326 groups. e Wnt1, Wnt3A, and β-catenin protein levels in tumors from blank, OE-CTRL, OE- LINC00326, shCTRL, and shLINC00326 groups. f–h Ki67 and TUNEL staining in blank, OE-CTRL, OE- LINC00326, shCTRL, and shLINC00326 groups, respectively (× 200, scale bars, 100 µm); n = 3. Data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001
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Targeting MUS81 sensitizes the anticancer effect of WEE1 inhibitor MK1775 in gastric cancer in vitro and in vivo. a and b MTT assay was performed to examine the effect of MK1775 on proliferation of MUS81 knockdown and parental gastric cancer cells SGC7901 and BGC823. Data are reported as mean ± SD for five independent experiments. c Clonogenic assay was performed to detect the anticancer effect of MK1775 in MUS81 knockdown and parental gastric cancer cells SGC7901 (left) and BGC823 (right). Representative images are displayed. d Data of the clonogenic assay in SGC7901 (left) and BGC823 (right) cells are reported as mean ± SD for three independent experiments. e The image of tumors in the SGC7901 gastric cancer xenograft mice model (n = 6 for each group) after treatment for 16 d. f Tumor growth curve of indicated groups in the SGC7901 gastric cancer xenograft mice model. The data are presented as mean ± SD (n = 6 for each group). g Tumor volume on day 16 after treatment. Data are presented as mean ± SD (n = 6 for each group). h Representative images of immunohistochemical (IHC) staining of Ki67 in the gastric cancer xenograft mice model. Scale bar: 25 μm. i IHC staining analysis of Ki 67-positive cells in different groups. The data are presented as mean ± SD (n = 3). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns. , not significant
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