Human BMP-2 PicoKine™ Quick ELISA Kit

Human BMP-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human BMP2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: FEK0311
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: bone tissue, cell culture supernatants and serum.
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human BMP-2 PicoKine™ Quick ELISA Kit

See all BMP2 products

SKU/Catalog Number

FEK0311

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human BMP-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human BMP2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human BMP-2 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0311)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0311 is reactive to BMP2 in Human samples

Validated Sample Types

bone tissue, cell culture supernatants and serum.

Application Guarantee

FEK0311 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity
FEK0311-CAP 96-well plate precoated with anti-Human BMP2 antibody 1
FEK0311-ST lyophilized recombinant Human BMP2 standard 10 ng/tube
FEK0311-DA Biotinylated anti-Human BMP2 detection antibody (50x) 130 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0020.0260.0600.1140.3490.8081.4522.489

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)552501005592551061
Standard deviation4.2913.2570.354.6615.5583.81
CV (%)7.8%5.3%7%7.9%6.1%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BMP2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 155515851532.945.5%
Sample 225024524027525213.465.3%
Sample 3100588588595293150.365.4%
*number of samples for each test n=16.

Gene/Protein Information For BMP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P12643

Gene ID

650

Gene Name

BMP2

Full Name

Bone morphogenetic protein 2

Weight

44.702kDa

Superfamily

TGF-beta family

Alternative Names

BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC BMP2 BDA2A, SSFSC, SSFSC1, BMP2 bone morphogenetic protein 2 bone morphogenetic protein 2|bone morphogenetic protein 2A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BMP2, check out the BMP2 Infographic

BMP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

FEK0311 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang Y, You C, Wei R, Zu J, Song C, Li J, Yan J. Biomed Res Int. 2017;2017:2971413. doi: 10.1155/2017/2971413. Epub 2017 Aug 17. Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified TAT Peptide to Enh...

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1 Customer Q&As for Human BMP-2 PicoKine™ Quick ELISA Kit

Question

Could you please tell me for how long at temperature -20/-80 Celsius this antibody Human BMP-2 PicoKine Fast ELISA Kit still stable?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

We do not set expiration dates on our products. Most of our un-conjugated antibodies are stable that last for several years if stored properly. We recommend that you follow the storage instruction for this Human BMP-2 PicoKine Fast ELISA Kit you purchased.

Boster Scientific Support

Answered: 2019-10-29

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