Anti-Lfc ARHGEF2 Antibody

Boster Bio Anti-Lfc ARHGEF2 Antibody catalog # A02572. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02572
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Lfc ARHGEF2 Antibody

See all GEF-H1 products

SKU/Catalog Number

A02572

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Lfc ARHGEF2 Antibody catalog # A02572. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lfc ARHGEF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02572)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human Lfc around the non-phosphorylation site of S885.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02572 is reactive to ARHGEF2 in Human, Mouse, Rat

Applications

A02572 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For ARHGEF2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q92974

Gene ID

9181

Gene Name

ARHGEF2

Full Name

Rho guanine nucleotide exchange factor 2

Weight

111.543kDa

Alternative Names

GEF; GEFH1; GEF-H1DKFZp547P1516; Guanine nucleotide exchange factor H1; KIAA0651DKFZp547L106; LFP40FLJ45440; Microtubule-regulated Rho-GEF; P40; Proliferating cell nucleolar antigen p40; rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2; Rho/Rac guanine nucleotide exchange factor 2 ARHGEF2 GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40 Rho/Rac guanine nucleotide exchange factor 2 rho guanine nucleotide exchange factor 2|Rho/Rac guanine nucleotide exchange factor (GEF) 2|guanine nucleotide exchange factor H1|microtubule-regulated Rho-GEF|proliferating cell nucleolar antigen p40

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ARHGEF2, check out the ARHGEF2 Infographic

ARHGEF2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ARHGEF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-Lfc ARHGEF2 Antibody

Question

Does A02572 anti-Lfc antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

It shows on the product datasheet, A02572 anti-Lfc antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-29

Question

My question regarding product A02572, anti-Lfc antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02572 anti-Lfc antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-01

Question

Is this A02572 anti-Lfc antibody reactive to the isotypes of ARHGEF2?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

The immunogen of A02572 anti-Lfc antibody is Synthesized peptide derived from human Lfc around the non-phosphorylation site of S885. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-27

Question

Is a blocking peptide available for product anti-Lfc antibody (A02572)?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

We do provide the blocking peptide for product anti-Lfc antibody (A02572). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-05

Question

We are currently using anti-Lfc antibody A02572 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

H. Walker

Verified customer

Asked: 2013-06-25

Answer

The anti-Lfc antibody (A02572) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-06-25

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A02572
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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