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Product Info Summary
| SKU: | A02572 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Lfc ARHGEF2 Antibody
SKU/Catalog Number
A02572
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Lfc ARHGEF2 Antibody catalog # A02572. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lfc ARHGEF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02572)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from C-ternal human ARHGEF2. AA range:851-900
Reactive Species
A02572 is reactive to ARHGEF2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
111.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02572 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of HT29 cells using Lfc Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from NIH/3T3 cells, using Rho/Rac Guanine Nucleotide Exchange Factor 2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Lfc ARHGEF2 Antibody (A02572)
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5 Customer Q&As for Anti-Lfc ARHGEF2 Antibody
Question
Does A02572 anti-Lfc antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
It shows on the product datasheet, A02572 anti-Lfc antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-29
Question
My question regarding product A02572, anti-Lfc antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02572 anti-Lfc antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-01
Question
Is this A02572 anti-Lfc antibody reactive to the isotypes of ARHGEF2?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The immunogen of A02572 anti-Lfc antibody is Synthesized peptide derived from human Lfc around the non-phosphorylation site of S885. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-27
Question
Is a blocking peptide available for product anti-Lfc antibody (A02572)?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We do provide the blocking peptide for product anti-Lfc antibody (A02572). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-05
Question
We are currently using anti-Lfc antibody A02572 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
H. Walker
Verified customer
Asked: 2013-06-25
Answer
The anti-Lfc antibody (A02572) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-25
