Anti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband®

Product Name

Anti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband®

View all LASP1 Antibodies

SKU/Catalog Number

PA1791
BA3755 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LIM and SH3 domain protein 1 LASP1 Antibody catalog # PA1791. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1791)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human LASP1, identical to the related rat sequence and different from the related mouse sequence by one amino acid.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1791 is reactive to LASP1 in Human, Mouse, Rat

Observed Molecular Weight

11 kDa

Calculated molecular weight

29.7 kDa

Background of LASP1

LASP1 (LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al. (1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1791 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: Rat Liver Tissue, Rat Spleen Tissue, Rat Intestine Tissue, JURKAT Cell, MCF-7 Cell, A431 Cell, HELA Cell, 293T Cell
IHC: Human Lung Cancer Tissue
ICC: A549 Cell

Validation Images & Assay Conditions

See full target information LASP1

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