Anti-LIM and SH3 domain protein 1 LASP1 Antibody

LASP1 antibody

Boster Bio Anti-LIM and SH3 domain protein 1 LASP1 Antibody catalog # PA1791-1. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PA1791-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-LIM and SH3 domain protein 1 LASP1 Antibody

View all LASP1 Antibodies

SKU/Catalog Number

PA1791-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LIM and SH3 domain protein 1 LASP1 Antibody catalog # PA1791-1. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LIM and SH3 domain protein 1 LASP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1791-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LASP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1791-1 is reactive to LASP1 in Human

Applications

PA1791-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

11 kDa

Calculated molecular weight

29.717kDa

Background of LASP1

LASP1 (LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al. (1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For LASP1 (Source: Uniprot.org, NCBI)

Gene Name

LASP1

Full Name

LIM and SH3 domain protein 1

Weight

29.717kDa

Alternative Names

LASP-1; LIM and SH3 domain protein 1; LIM and SH3 protein 1; Metastatic lymph node gene 50 protein; MLN 50; MLN50Lasp-1 LASP1 Lasp-1, MLN50 LIM and SH3 protein 1 LIM and SH3 domain protein 1|metastatic lymph node gene 50 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LASP1, check out the LASP1 Infographic

LASP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LASP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1791-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lu C,Fu L,Qian X,Dou L,Cang S.Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis.Arch Biochem Biophys.2020 Aug 15;689:108434.doi:10.1016/j.abb.2020.108434.Epub 2020 May 29.PMID:32473899
Species: Human,Mouse
PA1791-1 usage in article: APP:WB, SAMPLE:HCT116 CELL AND SW480 CELL, DILUTION:NA

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4 Customer Q&As for Anti-LIM and SH3 domain protein 1 LASP1 Antibody

Question

Is a blocking peptide available for product anti-LASP1 antibody (PA1791-1)?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

We do provide the blocking peptide for product anti-LASP1 antibody (PA1791-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-05

Question

See below the WB image, lot number and protocol we used for liver using anti-LASP1 antibody PA1791-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-07-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-19

Question

We are currently using anti-LASP1 antibody PA1791-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-30

Answer

The anti-LASP1 antibody (PA1791-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-30

Question

Can you help my question with product PA1791-1, anti-LASP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

N. Baker

Verified customer

Asked: 2014-04-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1791-1 anti-LASP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-04-21

Order DetailsPrice
PA1791-1

100μg

$370
PA1791-1-10ug

10μg sample (liquid)

$99
PA1791-1-Biotin

100 μg Biotin conjugated

$570
PA1791-1-Cy3

100 μg Cy3 conjugated

$570
PA1791-1-Dylight488

100 μg Dylight488 conjugated

$570
PA1791-1-Dylight550

100 μg Dylight550 conjugated

$570
PA1791-1-Dylight594

100 μg Dylight594 conjugated

$570
PA1791-1-FITC

100 μg FITC conjugated

$570
PA1791-1-HRP

100 μg HRP conjugated

$570
PA1791-1-APC

100 μg APC conjugated

$670
PA1791-1-PE

100 μg PE conjugated

$670
PA1791-1-carrier-free

Carrier Free

$370

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PA1791-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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