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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-TXNL2/GLRX3 Antibody Picoband™
Boster Bio Anti-TXNL2/GLRX3 Antibody Picoband™ catalog # PB10066. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TXNL2/GLRX3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10066)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human TXNL2 recombinant protein (Position: L89-S177). Human TXNL2 shares 93.3% and 94.4% amino acid (aa) sequence identity with mouse and rat TXNL2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
PB10066 is reactive to GLRX3 in Human, Mouse, Rat
PB10066 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TXNL2 using anti-TXNL2 antibody (PB10066).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates，
Lane 2: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNL2 antigen affinity purified polyclonal antibody (Catalog # PB10066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNL2 at approximately 40 kDa. The expected band size for TXNL2 is at 37 kDa.
Gene/Protein Information For GLRX3 (Source: Uniprot.Org, NCBI)
FLJ11864; GLRX3; GLRX4; Glutaredoxin 3; glutaredoxin 4; glutaredoxin 5; GRX3; GRX4; PICOT; PICOTbA500G10.4; PKC-interacting cousin of thioredoxin; PKCq-interacting protein; thioredoxin-like 2; TXNL2; TXNL3 GLRX3 GLRX4, GRX3, GRX4, PICOT, TXNL2, TXNL3 glutaredoxin 3 glutaredoxin-3|PKC-interacting cousin of thioredoxin|PKC-theta-interacting protein|PKCq-interacting protein|glutaredoxin 4|testicular tissue protein Li 75|thioredoxin-like protein 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on GLRX3, check out the GLRX3 Infographic
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In this infographic you will see the following information for GLRX3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB10066
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