Click image to see more details
Product Info Summary
| SKU: | PB9270 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-LKB1/STK11 Antibody Picoband®
SKU/Catalog Number
PB9270
PB0257 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LKB1/STK11 Antibody Picoband® catalog # PB9270. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LKB1/STK11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9270)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LKB1 recombinant protein (Position: K62-C430). Human LKB1 shares 89% amino acid (aa) sequence identity with both mouse and rat LKB1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9270 is reactive to STK11 in Human
Observed Molecular Weight
55 kDa
Calculated molecular weight
48.6 kDa
Background of STK11
Serine/threonine kinase 11 or LKB1 is a protein kinase which in humans is encoded by the STK11 gene. The STK11 gene, which encodes a member of the serine/threonine kinase, regulates cell polarity and functions as a tumour suppressor. STK11 is a primary upstream kinase of adenine monophosphate-activated protein kinase (AMPK), a necessary element in cell metabolism that is required for maintaining energy homeostasis. It has been found that LKB1 physically associates with p53 and regulates specific p53-dependent apoptosis pathways. The STK11 gene extends over 23 kb of genomic DNA and is composed of 9 exons, which are transcribed in telomere-to-centromere direction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9270 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human HepG2 whole cell, human K562 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of LKB1/STK11 using anti-LKB1/STK11 antibody (PB9270).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LKB1/STK11 antigen affinity purified polyclonal antibody (PB9270) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LKB1/STK11 at approximately 55 kDa. The expected band size for LKB1/STK11 is at 49 kDa.
Specific Publications For Anti-LKB1/STK11 Antibody Picoband® (PB9270)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-LKB1/STK11 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-LKB1/STK11 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-LKB1/STK11 Antibody Picoband®
Question
We are currently using anti-LKB1/STK11 antibody PB9270 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The anti-LKB1/STK11 antibody (PB9270) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-03
Question
I was wanting to use using your anti-LKB1/STK11 antibody for negative regulation of cell growth studies. Has this antibody been tested with western blotting on 293t whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
We appreciate your inquiry. This PB9270 anti-LKB1/STK11 antibody is validated on human placenta tissue, 293t whole cell lysate, ht1080 whole cell lysate, shg whole cell lysate, jurkat whole cell lysate, tissue lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-16
Question
We have been able to see staining in human brain. What should we do? Is anti-LKB1/STK11 antibody supposed to stain brain positively?
D. Evans
Verified customer
Asked: 2018-02-05
Answer
According to literature brain does express STK11. According to Uniprot.org, STK11 is expressed in testis, liver, brain, lung uterus, renal cell carcinoma, erythroleukemia, among other tissues. Regarding which tissues have STK11 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 9425897, 24275569
Lung, and Uterus, Pubmed ID: 15489334
Renal cell carcinoma, Pubmed ID: 10508479, 11212897
Boster Scientific Support
Answered: 2018-02-05
Question
My colleagues were satisfied with the WB result of your anti-LKB1/STK11 antibody. However we have observed positive staining in renal cell carcinoma isoform 2: nucleus using this antibody. Is that expected? Could you tell me where is STK11 supposed to be expressed?
A. Zhang
Verified customer
Asked: 2014-06-20
Answer
From what I have seen in literature, renal cell carcinoma does express STK11. Generally STK11 expresses in nucleus. cytoplasm. membrane., isoform 2: nucleus. Regarding which tissues have STK11 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 9425897, 24275569
Lung, and Uterus, Pubmed ID: 15489334
Renal cell carcinoma, Pubmed ID: 10508479, 11212897
Boster Scientific Support
Answered: 2014-06-20
