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Product Info Summary
| SKU: | PA2273 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LRP5 Antibody Picoband®
SKU/Catalog Number
PA2273
BA4305-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LRP5 Antibody catalog # PA2273. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LRP5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2273)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human LRP5, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2273 is reactive to LRP5 in Human, Mouse, Rat
Observed Molecular Weight
179 kDa
Calculated molecular weight
179.1 kDa
Background of LRP5
Low-density lipoprotein receptor-related protein 5 is a protein that in humans is encoded by the LRP5 gene. It is mapped to 11q13.2. LRP5 is a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with diabetes mellitus type 1 in humans. This protein plays a key role in skeletal homeostasis. In addition to that, the binding of axin to LRP5 is an important part of the Wnt signal transduction pathway, and it also acts as a target for the inhibitory effects of Dickkopf, another developmental protein, on Wnt signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2273 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Liver Tissue, Mouse Liver Tissue, NIH Whole Cell
Validation Images & Assay Conditions
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Anti-LRP5 antibody, PA2273, All Western blotting
All lanes: Anti-LRP5(PA2273) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Predicted bind size: 179KD
Observed bind size: 179KD
Specific Publications For Anti-LRP5 Antibody Picoband® (PA2273)
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Customer Q&As
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6 Customer Q&As for Anti-LRP5 Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for osteoblast using anti-LRP5 antibody PA2273. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-LRP5 antibody PA2273 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The anti-LRP5 antibody (PA2273) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-12
Question
We want to test anti-LRP5 antibody PA2273 on human osteoblast for research purposes, then I may be interested in using anti-LRP5 antibody PA2273 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The products we sell, including anti-LRP5 antibody PA2273, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this PA2273 anti-LRP5 antibody reactive to the isotypes of LRP5?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The immunogen of PA2273 anti-LRP5 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human LRP5(1201-1215aa IHAVEEVSLEEFSAH), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-04
Question
I see that the anti-LRP5 antibody PA2273 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-24
Question
I have a question about product PA2273, anti-LRP5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2273 anti-LRP5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-27
