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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Estrogen Receptor beta/ESR2 Antibody Picoband™
Boster Bio Anti-Estrogen Receptor beta/ESR2 Antibody Picoband™ catalog # A00786-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Estrogen Receptor beta/ESR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00786-1)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human ESR2 recombinant protein (Position: F289-R501). Human ESR2 shares 93.4% and 93.9% amino acid (aa) sequence identity with mouse and rat ESR2, respectively.
No cross reactivity with other proteins.
A00786-1 is reactive to ESR2 in Human, Mouse, Rat
A00786-1 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysate,
Lane 2: rat ovary tissue lysate,
Lane 3: mouse testis tissue lysate,
Lane 4: mouse ovary tissue lysate,
Lane 5: human Hela whole Cell lysate,
Lane 6: human MCF-7 whole Cell lysate,
Lane 7: human MDA-MB-231 whole Cell lysate,
Lane 8: human MDA-MB-453 whole Cell lysate,
Lane 9: human SK-OV-3 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59KD. The expected band size for ESR2 is at 59KD.
Gene/Protein Information For ESR2 (Source: Uniprot.Org, NCBI)
Estrogen receptor beta
nuclear hormone receptor family
ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2 ESR2 ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8 estrogen receptor 2 estrogen receptor beta|estrogen receptor beta 4|estrogen receptor beta splice variant, ERbeta2delta7|estrogen receptor beta splice variant, ERbeta4delta7|estrogen receptor beta splice variant, ERbeta6|estrogen receptor beta splice variant, ERbeta6delta7|estrogen receptor beta splice variant, ERbeta7|estrogen receptor beta splice variant, ERbeta7delta7|estrogen receptor beta splice variant, ERbetaEx. 4L|estrogen receptor beta splice variant, ERbetaEx. 6L|nuclear receptor subfamily 3 group A member 2|oestrogen receptor beta*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ESR2, check out the ESR2 Infographic
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In this infographic you will see the following information for ESR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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