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Product Info Summary
| SKU: | A00786-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Estrogen Receptor beta/ESR2 Antibody Picoband®
SKU/Catalog Number
A00786-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Estrogen Receptor beta/ESR2 Antibody Picoband® catalog # A00786-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Estrogen Receptor beta/ESR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00786-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ESR2 recombinant protein (Position: F289-R501). Human ESR2 shares 93.4% and 93.9% amino acid (aa) sequence identity with mouse and rat ESR2, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00786-1 is reactive to ESR2 in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.2 kDa
Background of ESR2
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00786-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human PC-3 whole cell, human MCF-7 whole cell, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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The expression of ESR2 (A) and AR (B) in the renal tissues from the control group, oxalate group, oxalate + low-dose GS group, and oxalate + high-dose GS group detected using IHC staining.
Index in PubMed under a CC BY license. PMID: 34248618
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Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
Specific Publications For Anti-Estrogen Receptor beta/ESR2 Antibody Picoband® (A00786-1)
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