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- Table of Contents
IL16 antibody (PB9238) performs well in ELISA, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Anti-IL-16 antibody, PB9238, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
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Anti-IL16 Antibody Picoband®
IL16 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC, ICC, WB |
Tested Applications
IL16 antibody was positively detected by WB in:
human U937 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
IL16 antibody was positively detected by IHC in:
Human Tonsil Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
IL16 antibody was positively detected by ICC in:
human cord blood
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry
0.5-1μg/ml
Human, -
Western blot
0.1-0.5μg/ml
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-IL16 Antibody Picoband®
View all IL16 Antibodies
View all IL16 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IL-16 recombinant protein (Position: S1212-S1332). Human IL-16 shares 86% amino acid (aa) sequence identity with mouse IL-16.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45-55 kDa
Calculated Molecular Weight
141.8 kDa
Background of IL16
Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. It is mapped to 15q25.1. IL-16 was originally described as a factor that could attract activated T cells in humans. It was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16 stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
Cite This Product
Anti-IL16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9238)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Mouse
86%
Moderate Homology
Cow
86%
Moderate Homology
Sheep
86%
Moderate Homology
Dog
85%
Moderate Homology
Rat
80%
Moderate Homology
Rabbit
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IL16 antibody
Immunohistochemistry (IHC) protocol for IL16 antibody
Immunocytochemistry (ICC) protocol for IL16 antibody
Publications
Application:
Species:
microRNA-128-3p inhibits CD4+ regulatory T cells enrichment by targeting interleukin 16 in gastric cancer
Published: 2021-12-30
Journal: Bioengineered
Application: IHC
Impact Factor: 3.27
Species: Human
PMID: 34968167
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Product Promise
We promise Anti-IL16 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9238
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026