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Product Info Summary
| SKU: | M00221-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LRRK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00221-2
BM4025 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LRRK2 Rabbit Monoclonal Antibody catalog # M00221-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LRRK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00221-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AGB-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LRRK2
Reactive Species
M00221-2 is reactive to LRRK2 in Human, Mouse, Rat
Observed Molecular Weight
280 kDa
Calculated molecular weight
286.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00221-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: U87-MG cell
IHC: human thyroid tissue
Validation Images & Assay Conditions
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Western blot analysis of LRRK2 expression in U87-MG cell lysate.
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Immunohistochemical analysis of paraffin-embedded human thyroid, using LRRK2 Antibody.
Specific Publications For Anti-LRRK2 Rabbit Monoclonal Antibody (M00221-2)
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4 Customer Q&As for Anti-LRRK2 Rabbit Monoclonal Antibody
Question
We have seen staining in mouse brain. What should we do? Is anti-LRRK2 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
From literature brain does express LRRK2. From Uniprot.org, LRRK2 is expressed in visceral pleura, brain, testis, liver, among other tissues. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-12-04
Question
We are currently using anti-LRRK2 Rabbit Monoclonal antibody M00221-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The anti-LRRK2 Rabbit Monoclonal antibody (M00221-2) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-19
Question
Our team were satisfied with the WB result of your anti-LRRK2 Rabbit Monoclonal antibody. However we have seen positive staining in visceral pleura cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is LRRK2 supposed to be expressed?
J. Kulkarni
Verified customer
Asked: 2018-11-28
Answer
Based on literature, visceral pleura does express LRRK2. Generally LRRK2 expresses in cytoplasmic vesicle. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2018-11-28
Question
We ordered your anti-LRRK2 Rabbit Monoclonal antibody for IHC on visceral pleura in a previous project. I am using mouse, and We are going to use the antibody for IF next. My question regards examining visceral pleura as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
B. Parker
Verified customer
Asked: 2016-02-02
Answer
I have checked the website and datasheets of our anti-LRRK2 Rabbit Monoclonal antibody and it appears that M00221-2 has been tested on mouse in both IHC and IF. Thus M00221-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-02-02
