Anti-LRRK2 Rabbit Monoclonal Antibody

Lrrk2 antibody

Boster Bio Anti-LRRK2 Rabbit Monoclonal Antibody catalog # M00221-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00221-2
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-LRRK2 Rabbit Monoclonal Antibody

View all Lrrk2 Antibodies

SKU/Catalog Number

M00221-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-LRRK2 Rabbit Monoclonal Antibody catalog # M00221-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LRRK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00221-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AGB-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human LRRK2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00221-2 is reactive to Lrrk2 in Human, Mouse, Rat

Applications

M00221-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

284.732kDa

Background of Lrrk2

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For Lrrk2 (Source: Uniprot.org, NCBI)

Gene Name

Lrrk2

Full Name

Leucine-rich repeat serine/threonine-protein kinase 2

Weight

284.732kDa

Superfamily

protein kinase superfamily

Alternative Names

augmented in rheumatoid arthritis 17; AURA17; Dardarin; DKFZp434H2111; EC 2.7.11.1; FLJ45829; leucine-rich repeat kinase 2; leucine-rich repeat serine/threonine-protein kinase 2; LRRK2; PARK8; PARK8DARDARIN; Parkinson disease (autosomal dominant) 8; RIPK7; ROCO2 Lrrk2|4921513O20Rik, 9330188B09Rik, AW561911, D630001M17Rik, Gm927, cI-4, cI-46|leucine-rich repeat kinase 2|leucine-rich repeat serine/threonine-protein kinase 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Lrrk2, check out the Lrrk2 Infographic

Lrrk2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Lrrk2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-LRRK2 Rabbit Monoclonal Antibody

Question

We have seen staining in mouse brain. What should we do? Is anti-LRRK2 Rabbit Monoclonal antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

From literature brain does express LRRK2. From Uniprot.org, LRRK2 is expressed in visceral pleura, brain, testis, liver, among other tissues. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2019-12-04

Question

We are currently using anti-LRRK2 Rabbit Monoclonal antibody M00221-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The anti-LRRK2 Rabbit Monoclonal antibody (M00221-2) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-19

Question

Our team were satisfied with the WB result of your anti-LRRK2 Rabbit Monoclonal antibody. However we have seen positive staining in visceral pleura cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is LRRK2 supposed to be expressed?

J. Kulkarni

Verified customer

Asked: 2018-11-28

Answer

Based on literature, visceral pleura does express LRRK2. Generally LRRK2 expresses in cytoplasmic vesicle. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2018-11-28

Question

We ordered your anti-LRRK2 Rabbit Monoclonal antibody for IHC on visceral pleura in a previous project. I am using mouse, and We are going to use the antibody for IF next. My question regards examining visceral pleura as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

B. Parker

Verified customer

Asked: 2016-02-02

Answer

I have checked the website and datasheets of our anti-LRRK2 Rabbit Monoclonal antibody and it appears that M00221-2 has been tested on mouse in both IHC and IF. Thus M00221-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-02-02

Order DetailsPrice
M00221-2

100μL

$370
M00221-2-30ul

30μL sample

$99
M00221-2-carrier-free

Carrier Free

$370

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