Anti-STUB1 Antibody Picoband™ (monoclonal, 13I8)

CHIP/STUB1 antibody

Boster Bio Anti-STUB1 Antibody Picoband™ (monoclonal, 13I8) catalog # M01236. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Info Summary

SKU: M01236
Size: 100μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IHC, WB

Product Name

Anti-STUB1 Antibody Picoband™ (monoclonal, 13I8)

View all CHIP/STUB1 Antibodies

SKU/Catalog Number

M01236

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-STUB1 Antibody Picoband™ (monoclonal, 13I8) catalog # M01236. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-STUB1 Antibody Picoband™ (monoclonal, 13I8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01236)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

13I8

Isotype

IgG2b

Immunogen

E.coli-derived human STUB1 recombinant protein (Position: R51-Y303).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01236 is reactive to STUB1 in Human, Monkey, Mouse, Rat

Applications

M01236 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Background of CHIP/STUB1

STUB1 (STIP1 homology and U-Box containing protein 1), also known as CHIP (C terminus of HSC70-Interacting Protein), is a human gene. This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For STUB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9UNE7

Gene ID

10273

Gene Name

STUB1

Full Name

E3 ubiquitin-protein ligase CHIP

Weight

34.856kDa

Alternative Names

Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CHIPSTIP1 homology and U box-containing protein 1; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; EC 6.3.2.-; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STUB1; UBOX1 STUB1 CHIP, HSPABP2, NY-CO-7, SCA48, SCAR16, SDCCAG7, UBOX1 STIP1 homology and U-box containing protein 1 E3 ubiquitin-protein ligase CHIP|CLL-associated antigen KW-8|RING-type E3 ubiquitin transferase CHIP|STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase|antigen NY-CO-7|carboxy terminus of Hsp70-interacting protein|heat shock protein A binding protein 2 (c-terminal)|serologically defined colon cancer antigen 7

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on STUB1, check out the STUB1 Infographic

STUB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for STUB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M01236

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6 Customer Q&As for Anti-STUB1 Antibody Picoband™ (monoclonal, 13I8)

Question

Is this M01236 anti-STUB1 antibody (monoclonal, 13I8) reactive to the isotypes of STUB1?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

The immunogen of M01236 anti-STUB1 antibody (monoclonal, 13I8) is E.coli-derived human STUB1 recombinant protein (Position: R51-Y303). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-22

Question

Does anti-STUB1 antibody (monoclonal, 13I8) M01236 work for Flow Cytometry with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

According to the expression profile of cervix carcinoma erythroleukemia, STUB1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-STUB1 antibody (monoclonal, 13I8) M01236 will work for Flow Cytometry with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2019-08-05

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-STUB1 antibody (monoclonal, 13I8) M01236. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-02-07

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-07

Question

I see that the anti-STUB1 antibody (monoclonal, 13I8) M01236 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-08-28

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-08-28

Question

Would anti-STUB1 antibody (monoclonal, 13I8) M01236 work on feline WB with heart?

Verified Customer

Verified customer

Asked: 2018-08-21

Answer

Our lab technicians have not tested anti-STUB1 antibody (monoclonal, 13I8) M01236 on feline. You can run a BLAST between feline and the immunogen sequence of anti-STUB1 antibody (monoclonal, 13I8) M01236 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline heart in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-21

Question

We are currently using anti-STUB1 antibody (monoclonal, 13I8) M01236 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

N. Jones

Verified customer

Asked: 2013-01-28

Answer

The anti-STUB1 antibody (monoclonal, 13I8) (M01236) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-01-28

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:M01236

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M01236
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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