Anti-LXR alpha/NR1H3 Antibody
Rabbit IgG polyclonal antibody for Oxysterols receptor LXR-alpha(NR1H3) detection. Tested with WB in Human;Mouse;Rat. Cited in 2 publication(s).
Anti-LXR alpha/NR1H3 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
MAF1 resembles Maf1 which is a Saccharomyces cerevisiae
|Product Name||Anti-LXR alpha/NR1H3 Antibody
See all NR1H3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for LXR alpha/NR1H3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. LXR alpha/NR1H3 information: Molecular Weight: 50396 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.|
|Cite This Product||Anti-LXR alpha/NR1H3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1246)|
|Similar Products From
|Anti-LXR alpha/NR1H3 Antibody may replace the following items: sc 14719|sc 14720.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human LXR alpha(375-398aa QVERLQHTYVEALHAYVSINHPHD), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For NR1H3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||10062|
|Species Of This Entry||Human|
|Protein Name||Oxysterols receptor LXR-alpha|
|Superfamily||nuclear hormone receptor family|
|Alternative Names||NR1H3|alpha; Liver X receptor alpha; liver X receptor-alpha; LXR alpha; LXRA; NR1H3; Nuclear receptor subfamily 1 group H member 3; nuclear receptor subfamily 1, group H, member 3; oxysterols receptor LXR-alpha; RLD-1|
|Post Tranlational Modifications||Phosphorylation; Hydroxylation; Cleavage|
|Subcellular Localization||Nucleus. Cytoplasm.|
|Gene Location||11p11.2, on Chromasome 11, gene sequence: NC_000011.10 (47248300..47269033)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For NR1H3 (Source: Uniprot.org, NCBI)
|Protein Function||Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (By similarity). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed:19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity).|
|Tissue Specificity||Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.|
|Interacting Proteins||EDF1; KDM1A; MDFI; NCOA1; NCOR1; PPARA; PPARD; PPARG; RXRA; RXRB; RXRG; SUV39H1|
|Research Areas||Cancer; Cholesterol Metabolism; Chromatin Research; Diabetes Research; Lipid and Metabolism; Signal Transduction; Transcription Factors and Regulators|
|Related Diseases||Diabetes Mellitus; Cardiovascular Diseases; Inflammation; Malignant Neoplasms; Hypercholesterolemia; Steatosis; Diabetes Mellitus, Non-insulin-dependent; Atherosclerosis; Dyslipidemias; Pre-eclampsia; Cell Invasion; Insulin Sensitivity; Obesity; Alzheimer's Disease; Cholangiocarcinoma; Coronary Heart Disease; Pregnancy Complications; Melanoma; Neoplasms, Experimental; Persistant Generalized Lymphadenopathy|
|Related Pathways||Cholesterol Homeostasis; Transport; Cholesterol Efflux; Cholesterol Transport; Reverse Cholesterol Transport; Lipid Homeostasis; Secretion; Aging; Excretion; Response To Lipid; Gluconeogenesis; Keratinocyte Differentiation; Cell Cycle Arrest; Tissue Development; Reverse Transcription; Cell Cycle; Response To Cholesterol; Hormone Secretion; Luteolysis; Cell Proliferation|
|Background||LXRA is a tissue-specific cofactor that permits RXRA to function as a potent 9cRA receptor with a distinct target gene specificity. It specifically interacts with RXRA in vivo to form a functional heterodimer in which RXRA is the ligand-binding subunit. Additionally, LXR activity is critical for physiologic lipid metabolism and transport. LXRs are endogenous inhibitors of atherogenesis and are targets for therapeutic intervention in cardiovascular disease. Furthermore, LXRs and their ligands are negative regulators of macrophage inflammatory gene expression. LXR is also found that as a transcriptional switch that integrates hepatic glucose metabolism and fatty acid synthesis. The LXR-IDOL-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake.|
|Scientific References||PMID: 7744246 by Willy P.J., et al. LXR, a nuclear receptor that defines a distinct retinoid response pathway.|
PMID: 19481530 by Jakobsson T., et al. GPS2 is required for cholesterol efflux by triggering histone demethylation, LXR recruitment, and coregulator assembly at the ABCG1 locus.
Our Boster Quality Guarantee for Anti-LXR alpha/NR1H3 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Liver Tissue Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: HELA Cell Lysate
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 2
|Shang W, Yu X, Wang H, Chen T, Fang Y, Yang X, Zhou P, Nie F, Zhou Q, Zhou J. Mol Med Rep. 2015 Jan;11(1):503-8. Doi: 10.3892/Mmr.2014.2731. Epub 2014 Oct 21. Fibroblast Growth Factor 21 Enhances Cholesterol Efflux In Thp-1 Macrophage-Derived Foam...||PubMed ID 25334019|
|Overexpression of C35 in breast carcinomas is associated with tumor progression and lymphnode metastasis||PubMed ID 26781796|
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Note: sample size takes longer to process, average 5-7 days.
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