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- Table of Contents
NR1H3 antibody (A03331-3) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of RT4 cells using anti-NR1H3 antibody (A03331-3).
Overlay histogram showing RT4 cells stained with A03331-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR1H3 Antibody (A03331-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing RT4 cells stained with A03331-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR1H3 Antibody (A03331-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-NR1H3 Antibody Picoband®
NR1H3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
NR1H3 antibody was positively detected by WB in:
human HepG2 whole cell, human RT4 whole cell, human Caco-2 whole cell, human A549 whole cell, rat C6 whole cell, rat RH35 whole cell, mouse Neuro-2a whole cell, mouse HEPA1-6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NR1H3 antibody was positively detected by FC in:
RT4 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Sheep
Predicted
Predicted
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-NR1H3 Antibody Picoband®
View all NR1H3 Antibodies
View all NR1H3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NR1H3 recombinant protein (Position: D11-R401). Human NR1H3 shares 91.3% and 90.5% amino acid (aa) sequence identity with mouse and rat NR1H3, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
50.4 kDa
Background of NR1H3
LXRA is a tissue-specific cofactor that permits RXRA to function as a potent 9cRA receptor with a distinct target gene specificity. It specifically interacts with RXRA in vivo to form a functional heterodimer in which RXRA is the ligand-binding subunit. Additionally, LXR activity is critical for physiologic lipid metabolism and transport. LXRs are endogenous inhibitors of atherogenesis and are targets for therapeutic intervention in cardiovascular disease. Furthermore, LXRs and their ligands are negative regulators of macrophage inflammatory gene expression. LXR is also found that as a transcriptional switch that integrates hepatic glucose metabolism and fatty acid synthesis. The LXR-IDOL-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake.
Cite This Product
Anti-NR1H3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03331-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Rabbit
92%
High Homology
Sheep
92%
High Homology
Cow
92%
High Homology
Dog
91%
High Homology
Mouse
91%
High Homology
Rat
91%
High Homology
Zebrafish
65%
Low homology
Drosophila
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NR1H3 antibody
Flow Cytometry (FC) protocol for NR1H3 antibody
Publications
Application:
Species:
3′-Hydroxypterostilbene Alleviates Obesity and Lipoprotein Metabolism Dysregulation by Reshaping Gut Microbiota in High-Fat Diet-Induced Obese Mice
Published: 2026-03-14
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 6.20
Species: Mouse
PMID: 41830637
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Product Promise
We promise Anti-NR1H3 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03331-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026