NR1H3 Antibodies

Oxysterols receptor LXR-alpha (NR1H3)

Nuclear receptor that shows a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding spouse to an active ligand-binding subunit in mediating retinoid responses through target genes characterized by LXRES (By similarity).

LXRES are DR4-type response components characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake via MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed:19481530).

Gene Information

Human
Gene Name:	NR1H3
Uniprot:	Q13133
Entrez:	10062

Family

Belongs to:
nuclear hormone receptor family

Alternative Names

alpha; Liver X receptor alpha; liver X receptor-alpha; LXR alpha; LXRA; NR1H3; Nuclear receptor subfamily 1 group H member 3; nuclear receptor subfamily 1, group H, member 3; oxysterols receptor LXR-alpha; RLD-1

Molecular Weight

Mass (kDA):
50.396 kDA

Genomic Context

Human
Location:	11p11.2
Sequence:	11; NC_000011.10 (47248300..47269033)

Tissue Specificity

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

Subcellular Localizations

Nucleus. Cytoplasm.

Diseases

• Diabetes Mellitus
• Cardiovascular Diseases
• Inflammation
• Malignant Neoplasms
• Hypercholesterolemia
• Steatosis
• Diabetes Mellitus, Non-insulin-dependent
• Atherosclerosis
• Dyslipidemias
• Pre-eclampsia
• Cell Invasion
• Insulin Sensitivity
• Obesity

• Alzheimer's Disease
• Cholangiocarcinoma
• Coronary Heart Disease
• Pregnancy Complications
• Melanoma
• Neoplasms, Experimental
• Persistant Generalized Lymphadenopathy

Interacting Proteins

• EDF1
• KDM1A
• MDFI
• NCOA1
• NCOR1

• PPARA
• PPARD
• PPARG
• RXRA
• RXRB

• RXRG
• SUV39H1

Pathways

• Cholesterol Homeostasis
• Transport
• Cholesterol Efflux
• Cholesterol Transport
• Reverse Cholesterol Transport
• Lipid Homeostasis
• Secretion
• Aging
• Excretion
• Response To Lipid
• Gluconeogenesis
• Keratinocyte Differentiation
• Cell Cycle Arrest

• Tissue Development
• Reverse Transcription
• Cell Cycle
• Response To Cholesterol
• Hormone Secretion
• Luteolysis
• Cell Proliferation

PTMs

• Phosphorylation

• Hydroxylation

• Cleavage

Research Areas

• Cancer
• Cholesterol Metabolism
• Chromatin Research
• Diabetes Research
• Lipid and Metabolism

• Signal Transduction
• Transcription Factors and Regulators

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NR1H3

Best Uses Of The NR1H3 Marker

There are many uses to NR1H3 antibodies. Boster provides antibodies that are high-affinity and have been validated on immunohistochemistry, immunoblotting, and ELISA. These antibodies have been used in many studies and are trusted by scientists. Here are some of the most popular uses for NR1H3 antibody. Continue reading for more information!

NR1H3 antibody is used in biological assays

NR1H3 a nuclear receptor that exhibits liganddependent transcriptional activation. Its interaction with retinoic acid receptors (RXRs) shifts their subunit from silent to active. The receptor mediates retinoic- acid responses via LXRES, DR4 type response elements with two identical hexanucleotide-half-sites. It interacts with the LDLR (VLDLR), and LRP8 to regulate cholesterol uptake. The NR1H3 proteins also interact with the RORA gene family, which is involved in liver metabolic.

TISSUES Experimental Tissue Protein Expression Evidence Scores identify tissues with high levels of NR1H3 protein expression. These scores were calculated from proteomics datasets. CTD Gene-Disease Associations database lists disease-associated genotypes containing NR1H3 proteins. TISSUES Textmining Tissue Protein expression Evidence Scores identify inter-acting drugs with NR1H3 pro.

The NR1H3 polymorphism can affect B cell proliferation assays. The RTPCR RNA products were separated on a 1% agarose jelly and stained with EtBr in ultraviolet illumination. Quantitative-PCR using bactin mRNA expression was used to measure mRNA levels and normalize for polymorphism. A paired t-test was used to calculate the P-value.

Biological assays involving NR1H3 have been essential in determining the role this gene plays in the disease process. Numerous studies have linked this gene with arthritis and atherosclerosis. The gene may also be involved in regulating inflammation and immune response in SLE patients. LXR Polymorphism may influence the immune response of SLE patients to the NR1H3 genetic gene.

ExAC has 21 people with the mutation MAF = 0.00002 and the disease in one or more of the affected biological relatives. These findings suggest that additional factors must be present for an individual to develop disease. The disease is highly heterogeneous and may require additional factors to trigger its onset. It is highly probable that MS is due to a genetic defect in the NR1H3.

Reactome databases, for example contains pathways involving NR1H3 genes. A comprehensive database containing NR1H3 gene expression data has been generated by the Roadmap Epigenomics Cell and TissueDNAMetholation Profiles and Highstone Modification Sites. This predicts NR1H3 -associated microRNAs. This information can help identify biologically relevant regulatory routes involving NR1H3's gene expression.

The LXR-alpha oxysterols receptor is a nuclear receptor

LXRs are central regulators for cholesterol and oxysterol metabolism. There are many neurodegenerative disorders that can be caused by their dysregulation. Animal models have shown that LXRs may reduce neuroinflammation and act neuroprotectively. There is an association between the encoding-gene polymorphisms for LXR and age at onset of Alzheimer's disease. LXR gene variants can increase the likelihood of developing neurodegenerative diseases, such as Huntington’s or Parkinson's. LXRs in cell culture may prevent synaptic defect.

LXRs regulate genes involved in lipid and glucose metabolism. LXRs can be activated to counter cellular sterol overload. They up-regulate expression of sterol transporters, transcription factors, and other genes involved in lipid and glucose metabolism. LXRs also inhibit LDL particle absorption by stimulating the production of an LDL-degrader inducer.

Studies have shown LXR activity is capable of regulating cholesterol and phospho-Tau levels within the CSF. LXRs also play an important role in controlling the metabolism cholesterol and oxysterols and are associated to familial forms of primary progressive MS. Moreover, LXRs are involved in the control of myelination. Thus, preventing inflammation is important.

LXR (oxysterol-activated LXR) induces macrophages to express ABCA1 and ABCG1. Because lipid-loaded macrophages are responsible for atherosclerosis' etiology, they can be used as a pharmacological target to sterol efflux. In addition, increased ABCA1 or ABCG1 expression in macrophages may limit cholesterol accumulation and protect against fatty lesions.

The nuclear receptors of the liver X are highly homologous transcription factors. They are closely related PPARs FXRs & RXRs. They regulate sterol, glucose, fatty acid and homeostasis. They also play a key role in the metabolic process for reverse cholesterol absorption.

LXRs have a broad antiinflammatory role in the body. They also suppress the expression pro-inflammatory genes. Trans-activation is LXRs’ most popular mode of action. LXRs could have been indirectly activated by small, ubiquitin-like protein fragments that were found within the receptor's target genes. This mechanism was later disproved by a number of studies.

LXR activation reduces pro-inflammatory cytokines maturation like IL-18, and inducing regulatory T cell expression. These two molecules also prevent macrophages from becoming inflamed. The LXRs also activate macrophage ABCA1 and inhibit pro-inflammatory T cell activity. LXRs have been a major target of immunotherapy and other medical studies.

LXRs are critical for cancer biology and the immune system. Oxysterols are cholesterol derivatives that can cross the blood-brain boundary. This crosstalk is essential for regulating the immune system and the development of inflammatory disorders. These specific pathways are involved in tumor progression, and modulating LXR activity may offer a new therapeutic approach.

Biological assays

Biological assays can be used to determine the effects of a drug on biological targets. NR1H3 inhibits proliferation of endometrial cancer cells by regulating the G1/S transition. Hence, it is important to understand its exact biological role in cancer research. Boster Bio's NR1H3 biological assays can be used for qualitative and quantitative testing.

Boster Biologicals offers a variety of antibody kits in varying formats and sensitivity levels. Boster Biologicals' antibodies have been tested against 250 different tissues and have high affinity. Boster Bio ELISA Kits are also quantified against known amounts of recombinant protein. Sanbio, a BeNeLux distributor provides technical support for the company's ELISA products.