Click image to see more details
Product Info Summary
| SKU: | M00785 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MAD2L1/Mad2 Monoclonal Antibody
SKU/Catalog Number
M00785
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-MAD2L1/Mad2 Monoclonal Antibody (Catalog # M00785). Tested in IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-MAD2L1/Mad2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00785)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 17D10 IgG1
Isotype
IgG1
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.
Reactive Species
M00785 is reactive to MAD2L1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
23.5 kDa
Background of MAD2L1
MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization. A MAD2 pseudogene has been mapped to chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00785 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:200 - 1:2,000
This protein A purified antibody has been tested for use in immunoprecipitation and by western blot. This antibody is not applicable for immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
Click image to see more details
Western blot using Boster's Mab anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate . Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Specific Publications For Anti-MAD2L1/Mad2 Monoclonal Antibody (M00785)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MAD2L1/Mad2 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-MAD2L1/Mad2 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
